Effect of Memory-enhancing Herbal Extract (YMT_02) on Modulating Pentraxin, PEP-19 and Transthyretin gene Expression in Rat Hippocampus

육미지황탕가미방에 의한 흰쥐 기억력 향상과 관련된 Hippocampus 부위의 특이 유전자 발현에 대한 연구

  • Sim Dea Sik (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Rho Sam Woong (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Lee Jin Woo (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Lee Eun A (Purimed R&D Institute) ;
  • Cho Chong Woon (Purimed R&D Institute) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyunghee University, Purimed R&D Institute) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, Kyunghee University)
  • 심대식 (경희대학교 한의과대학 생리학교실) ;
  • 노삼웅 (경희대학교 한의과대학 생리학교실) ;
  • 이진우 (경희대학교 한의과대학 생리학교실) ;
  • 이은아 (퓨리메드(주) 기업부설연구소) ;
  • 조종운 (퓨리메드(주) 기업부설연구소) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨리메드(주) 기업부설연구소) ;
  • 신민규 (경희대학교 한의과대학 생리학교실) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실)
  • Published : 2003.06.01

Abstract

The herbal extract(YMT_02) is a modified extracts from Yukmijihwang-tang(YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as replenishing yin and tonifying the kidneys herbal medicine for hundred years ia Asian countries. The purpose of this study is to: 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by passive avoidance test, 2) statistical evaluation of candidate gene expression (pentraxin. PEP-19, transthyretin) in rat hippocampus. The hippocampi of YMT_02 and control group were dissected and mRNA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Real Time PCR. The results were as follows : 1) passive avoidance test showed enhancing memory retentin by YMT_02 treatment, 2) expression of pentraxin, that accelerate degenerating of neuronal cell, was significantly decreased, 3) the mRNA of genes that has been known to be associated with protecting neuronal cell degeneration, such as PEP-19 and transthyretin, were significantly increased upon YMT_02 treatment. From above results, the administration of YMT_02 which tonify the function of Kidneys could enhance the ability of memory and learning. In addition, the administration of YMT_02 enhance memory retention through modulating particular gene (pentraxin, PEP-19, transthyretin) expressions in hippocampu.

Keywords

References

  1. 심리학입문 장동환 등
  2. 심리학입문 장동환 등
  3. 경희대학교 논문집 v.19 no.2 神에 대한 연구 정창현;박찬국
  4. 동의생리병리학회지 v.15 no.3 五神에 관한 연구 최성욱(외)
  5. Henry Gleitman's 심리학 장현갑(외 공역)
  6. 동의생리학 대한동의생리학회(편)
  7. Princples of Neural Science(4th edition) Kanndel;Schwartz;Jessell
  8. 동의생리학회지 v.5 no.1 동의학에서의 인지과정에 대한 고찰 이충렬;홍무창
  9. 동의생리학회지 v.15 no.1 腎기능이 학습과 기억에 미치는 영향에 대한 실험적 연구 하태현;이진우;채한;홍무창
  10. 경희대학교 석사학위논문 숙지황이 기억과 망각 속도에 미치는 영향에 대한 연구 박승원
  11. 동의생리병리학회지 v.15 no.3 구기자가 백서의 기억능력 및 망각속도에 미치는 영향 이진우;배현수;홍무창;신민규
  12. 동의생리병리학회지 v.16 no.5 육미지황탕가미방이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향 최보업;배현수;신민규;홍무창
  13. Brain Research v.813 no.2 The in vitro neuronal toxicity of pentraxins associated with Alzheimer's disease brain lesions. Duong, T.;Acton, P. J.;Johanson, R. A.
  14. Journal of Neuroscience v.15 no.20 Calmodulin-binding pepptide PEP-19 modulates activation of Calmodulin kinase Ⅱ In situ Johnson, R. A.;Sarau, H. M.;Foley, J. J.;Slemmon, J. R.
  15. Journal of Neurochemistry v.63 no.1 Correlative regulation of nerve growth factor level and choline acetyltransferase activity by thyroxine in particular regions of infant rat brain. Hashimoto, Y.;Furukawa, S.;Omae, F.;Miyama, Y.;Hayashi, K.
  16. Braz J Med Biol Res. v.24 no.3 Effect of post-training injections of flumazenil into the amygdala, hippocampus and septum on retention of habituation and of inhibito교 avoidance in rats. Da Cunha, C.;Wolfman, C.;Huang, C. H.;Walz, R.;Koya,R.;Bianchin, M.;Medina, J. H.;Izquierdo, I.
  17. 동양학의 방법론 연구 윤길영
  18. 동의신계내과학 두호경
  19. 姜帝內經譯解(靈樞) 楊維傑(編)
  20. 黃帝內經譯解(素問) 楊維傑(編)
  21. 難經本義 滑壽
  22. 懸吐醫學入門 李挻
  23. 한방임상학 채인식
  24. 靈素節注類篇 章楠
  25. Henry Gleitman's 심리학 장현갑(외 공역)
  26. 학습심리학 이주용(역)
  27. 심리학 장현갑(외)
  28. 難經集注 王九思
  29. 靈樞經校繹(上) 河北醫學院
  30. 類經 類經飜譯推進會(譯)
  31. 病機彙論 沈頲
  32. 金置要略方論 仲景全書 張機
  33. 小兒藥證直訣(下) 錢乙
  34. 동의보감 허준
  35. 방약합편 황도연
  36. 濟陰綱目 武之望
  37. 千金要方 孫恩邈
  38. 醫方集解 汪認庵
  39. 醫門寶鑑 周命新
  40. 동의임상방제학 윤길영
  41. 申氏本草學 신길구
  42. 本草學 전국한의과대학 본초학교실
  43. 동의생리학회지 v.6 no.1 기억과 腎의 상관성에 대한 실험적 연구 이재희;신민규;김완희
  44. 동의생리학회지 v.15 no.2 심기능이 학습과 기억에 미치는 영향에 대한 실험적 연구 김성경, 홍무창
  45. Journal of Experimental Psychology v.38 Studies of fear as an scquerable drive, I. Fear as motivation and fear-reduction as reinforcement in the learning of new response. Miller, N. E.
  46. Peptides v.21 no.3 Lateralized learning and memory effects of angiotensin Ⅱl microinjected into the rat CAI hippocampal area. Belcheva, I.;Ternianov, A.;Ceorgiev, V.
  47. Neur Learn Mem v.73 no.1 Shuttle-box avoidance learning in mice, improvement by glucose combined with stimulant drugs. Sansone, M.;Battaglia, M;Pavone, F.
  48. Folia Med (Plovdiv). v.42 no.1 Effect of ACE-inhibitors on learning and memory processes in rats Nikolova, J. G.;Getova, D. P.;Nikolov, F. P.
  49. Zh Vyssh Nerv Deiat Im I P Pavlova. v.50 no.2 Is "scopolamine-induced amnesia" in rats the result of state-dependent learning? Trofimov, S. S.;Borlikova, G. G.;Kravchenko, E. V.;Inozemtsov, A. N.;Ostrovskaia, R. U.;Voronina, T. A.
  50. Zh Vyssh Nerv Deiat Im I P Pavlova. v.50 no.2 Is "scopolamine-induced amnesia" in rats the result of state-dependent learning? Trofimov, S. S.;Borlikova, G. G.;Kravchenko, E. V.;Inozemtsov, A. N.;Ostrovskaia, R. U.;Voronina, T. A.
  51. Eur Neuropsychopharmacol. v.10 no.4 The GABA-B antagonist CGP 36742 prevent PTZ-kindling-provoked amnesia in rats. Genkova-Papazova, M. G.;Petkova, B.;Shishkova, N.;Lazarova-Bakarova, M.
  52. Biol Pharm Bull. v.23 no.4 Focal cerebral ischemia-induced escape deficit in rats is ameliorated by a reversible inhibitor of monoamine oxidase-a: implications for a novel animal model of post-stroke depression. Kato, M.;Iwata, M.;Okamoto, M.;Ishii, T.;Narita, H.
  53. J Biol Chem. v.275 no.12 Ran-mediated nuclear export of the von Hippel-Lindau tumor suppressor protein occurs independently of its assembly with cullin-2. Groulx, I.;Bonicalzi, M. E;Lee, S.
  54. Mutat Res. v.466 no.2 Comparison of three different in vitro mutation assays used for the investigation of cytochrome P450-mediated mutagenicity of nitro-polycyclic aromatic hydrocarbons. Kappers, W. A.;van Och, F. M.;de Groene, E. M;Horbach, G. J.
  55. Dev Genes Evol. v.210 no.2 A recombinogenic targeting method to modify large-inserts for cis-regulatory analysis in transgenic mice, construction and expression of a 100-kb, zebrafish Hoxa-11b-lacZ reporter gene. Chiu, C. H.;Amemiya, C. T.;Carr, J. L.;Bhargava, J.;Hwang, J. K.;Shashikant, C. S., Ruddle, F. H.;Wagner, G. P.
  56. Neuron. v.14 no.3 Neuronal pentraxin, a secreted protein with homology to acute phase proteins of the immune system. Schlimgen, A. K.;Helms, 1. A.;Vogel, H.;Perin, M. S.
  57. Brain Res Bull. v.58 no.5 DMA array reveals altered gene expression in response to focal cerebral ischemia. Kim, Y.;Sohn, N.;Kang, C.;Soh, Y.
  58. Neurobiol Aging v.22 no.6 The pentraxins: possible role in Alzheimer's disease and other innate inflammatory diseases. McGeer, E. G.;Yasojima, K.;Schwab, C.;McGeer, P. L.
  59. Neuroreport v.17 Expression of PEP-19 inhibits apoptosis in PC12 cells. Erhardt, J. A.;Legos, J. J.;Johanson, R. A,;Slemmon, J. R.;Wang, X.
  60. Neuroscience v.86 no.4 PEP-19 immunohistochemistry defines the basal ganglia and associated structures in the adult human brain, and is dramatically reduced in Huntington's disease. Utal, A. K.;Stopka, A. L.;Roy, M.;Coleman, P. D.
  61. Mol Neurobiol v.22 no.1;2;3 Small proteins that modulate calmodulin-dependent signal transduction:effects of PEP-19, neuromodulin, and neurogranin on enzyme activation and cellular homeostasis. Slemmon, J. R.;Feng, B.;Erhardt, J. A.
  62. Brain Research Reviews v.17 no.2 Neuroendocline regulatory mechanisms in the choroid plexuscerebrospinal fluid system. Nilsson, C.;Lindvall-Axelsson, M.;Owman, C.
  63. FEBS Lett. v.425 no.2 Apolipoprotein E, Transthyretin and actin in the CSF of Alzheimer's patients: relation with the senile plaques and cytoskeleton biochemistry. Merched, A.;Serot, J. M.;Visvikis S.;Aguillon, D.;Faure, G.;Siest, G.
  64. J Neurol Neurosurg Psychiatry v.63 no.4 Cerebrospinal fluid transthyretin: aging and late onset Alzheimer's disease. Serot, J. M.;Christmann, D.;Dubost, T.;Couturier, M.
  65. Learn Mem. v.8 no.4 Identification of genes expressed in the amygdala during the formation of fear memory. Stork, O.;Stork, S.;Pape, H. C;Obata, K.
  66. Neurology v.47 no.6 Familial meningocerebrovascular amyloidosis, Hungarian type, with mutant transthyretin(TTR Asp18G1y). Garzuly, F.;Vidal, R.;Wisniewski, T.;Brittig, F.;Budka, H.
  67. Alan Parkin's 기억연구의 실제와 응용 이영애;박희경(공역)