Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Dojeokseungki-tang on the apoptosis of Leukemia Cell

도적승기탕이 백혈병세포의 Apoptosis에 미치는 효과

  • Park Min Chul (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Kwon Jin (Department of Health Administration, Kunjang College) ;
  • Jeong Han Sol (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee Kwang Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
  • 박민철 (우석대학교 한의과대학 병리학교실) ;
  • 권진 (군장대학교 보건행정과) ;
  • 정한솔 (우석대학교 한의과대학 병리학교실) ;
  • 이광규 (우석대학교 한의과대학 병리학교실)
  • Published : 2003.04.01
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Abstract

The purpose of this research was to investigate the anticancer effects of Dojeokseungki-tang(DJSKT) on the various leukemia cell lines. DJSKT treatment suppressed proliferation of cultured-HL60, Jurkat, L1210 cells and increased apoptosis of cultured-L1210, HL60, Molt4, Jurkat cells. DJSKT treatment induced apoptosis of Jurkat cells including the morphologic changes such as the 'ladder pattern' revealed by agarose gel electrophoresis of DNA in a dose-dependent manner. Administration of DJSKT induced apoptosis of transplanted-L1210 cells in vivo, and decreased of mitochondrial transmembrane potential of L 1210 and Jurkat cells in vitro. DJSKT treatment reduced the expression of bcl-2 proteins in Jurkat cells and increased ICE, c-myc, p53 mRNA expression in Molt4 cells. In conclusion, these results suggest that DJSKT might be usefully applied for anti-carcinogenic agent of leukemia.

Keywords

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