Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Induction of Apoptotic Cell Death by Sabaek-san Extract in Human Lung Cancer A549 Cells

사백산 추출물에 의한 인체 폐암세포의 Apoptosis 유도 기전에 관한 연구

  • Lee Jae Hun (Department of Biochemistry, College of Oriental Medicine, Dongeui University) ;
  • Kang Byong Ryeung (Department of Internal Medicine, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Kam Cheol Woo (Department of Internal Medicine, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Park Dong Il (Department of Internal Medicine, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Choi Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
  • 이재훈 (동의대학교 한의과대학 생화학교실) ;
  • 강병령 (동의대학교 한의과대학 폐계내과학교실 및 한의학연구소) ;
  • 감철우 (동의대학교 한의과대학 폐계내과학교실 및 한의학연구소) ;
  • 박동일 (동의대학교 한의과대학 폐계내과학교실 및 한의학연구소) ;
  • 최영현 (동의대학교 한의과대학 생화학교실)
  • Published : 2003.04.01
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Abstract

We investigated the effects of Sabaek-san (SBS) water extract on the growth of human lung carcinoma A549 cells. Upon treatment with SBS extract, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis. including condensation of chromatin. Flow cytometry analysis confirmed that SBS treatment increased populations of apoptotic-sub G1 phase. In addition. proteolytic cleavages of poly(ADP-ribose) polymerase and β-catenin protein were observed after treatment of SBS extract. These apoptotic effects of SBS in A549 cells were associated with marked inhibition of Bcl-2 and Bel-xL mRNA in a dose-dependent manner. however the levels of Bax expression were not affected, SBS treatment also induced a proteolytic activation of caspase-3. which is believed to play a central role In the apoptotic signaling pathway. The previous and present results indicated that SBS-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression and the induction of apoptosis.

Keywords

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