Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Benincasae Semen on Glucose Oxidase in Cultured Mouse Spinal Motor Neurons

척수운동신경세포에 있어서 Glucose Oxidase의 독성에 대한 동과의 영향

  • Published : 2003.04.01
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Abstract

It has been suggested that oxidative stress of reactive oxygen species(ROS) may play an important role in the pathogenesis of neurological disorder. The aim of this study was to elucidate the oxidative stress of glucose oxidase(GO) in the cultured mouse spinal motor neurons and the preventing effect of Benincasae Semen(BS) on ROS-induced neurotoxicity. Cytotoxic effect of GO and protective effect of BS were performed by MTT assay. 30mU/ml GO decreased cell viability in dose-and time-dependent mannner, and BS diminished GO-induced neurotoxicity in these cultures. From above the results, ROS such as GO has toxic effect, and herb extract of BS is very effective against GO-induced neurotoxicity in cultured spinal motor neurons of neonatal mouse.

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References

  1. Korean J Pharmacology v.31 The role of increased oxidative stress in the development of diabetic nephropathy. Jang TJ;Park H;Kim HS;Hong HN;Kim MK
  2. J Neurosci Res v.37 Oxygen radical-induced meurotoxicity in spinal cord neuron cultures Michikawa M;Lim KT;McLarnon JG;Kim SU https://doi.org/10.1002/jnr.490370109
  3. J Neurosci v.10 Excitatory amino acid and free radical formation may cooperate in the genesis of ischemia-induced neuronal damage. Pellegrini-Giampietro DE;Cherici G;Alesiani M;Carrla V;Moroni F https://doi.org/10.1523/JNEUROSCI.10-03-01035.1990
  4. J Neurochem v.59 Reactive oxygen species and the central nervous system. Halliwell B https://doi.org/10.1111/j.1471-4159.1992.tb10990.x
  5. Diabetes v.41 The impact of diabetes on the CNS. McCall AL https://doi.org/10.2337/diabetes.41.5.557
  6. Korean J Phys Anthrop v.8 Study on the effect of iron-chletor on oxygen radical-induced neurotoxicity. Park ST https://doi.org/10.11637/kjpa.1995.8.2.113
  7. J Biolo Chern v.264 Hyperglycemic can cause membrane lipid peroxidation and osmotic fragility in human red blood cells. Jain S. K.
  8. J Neurosci Res v.29 Oligodendroglial cell death induced by oxygen radicals and its protection by catalase. Kim Y.S.;Kim S.U. https://doi.org/10.1002/jnr.490290111
  9. Diabetologia v.36 Antioxidant treatment prevents the development of peripheral nerve dysfunction in streptozotocin-diabetic rats. Camerone NE;Cotter MA;Maxfield EK https://doi.org/10.1007/BF00400231
  10. Nature (London) v.336 NMDA receptors activate the arachidonic acid cascade system in striatal neurons. Dumuis A;Sebben M;Haynes L;Pin J-P;Bockaert J https://doi.org/10.1038/336068a0
  11. J Biol Chem v.259 Inhibition of oxidation of methyl linoleate in solution by vitamin E and vitamin C. Niki E;Saito T;Kawakami A;Kamiya Y
  12. J lmmun Methods v.65 Rapid colorimetric assay for the cellular growth and survival: Application to proliferation and cytotoxic assays. Mosmann T https://doi.org/10.1016/0022-1759(83)90303-4
  13. Toxic in Vitro v.2 Comparisons of two in vitro cytotoxicity assays-the neutral red and tetrazolium MTT tests. Borenfreund E;Babich H;Martin-Alguacil N https://doi.org/10.1016/0887-2333(88)90030-6
  14. Circ Res v.57 Appearance of superoxide anion radical in cerebral extracellular space during increased prostaglandin synthesis in cat. Kontos H;Wei E;Ellis E;Jenkins L;Povlishock J;Rowe G;Hess M https://doi.org/10.1161/01.RES.57.1.142
  15. Proc Soc Exp Biol Med v.196 Determination of superoxide dismutase activity by the polarographic method of catalytic currents in the cerebrospinal fluid of aging brain and neurologic degenerative diseases. Bracco F.;Scarpa M.;Rigo A.;Battistin L.
  16. 생약학회지 v.30 no.3 육미지황탕이 카드늄 중독된 흰쥐의 혈압에 미치는 영향 임종필;서은실;김훈;송영철
  17. 동의병리학회지 v.9 no.1 한의학적 진단모형에 따른 한방제제의 항암 및 면역 조절작용에 미치는 영향 안규석;최승훈;김정범;박종현