Vasorelaxation Effect of Butanol Fraction of Crataegi Fructus due to LC20 dephosphorylation via increase of Myosin Phosphophatase activity

산사 Butaol 분획이 PGF2$\alpha$-유도 혈관평활근수축의 억제에 미치는 신호전달 연구

  • Liang Liou Jia (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Choi Ho Jeong (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Kim Gil-Whon (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Shin Heung Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
  • 유가량 (동국대학교 한의과대학 생리학교실) ;
  • 최호정 (동국대학교 한의과대학 생리학교실) ;
  • 김길훤 (동국대학교 한의과대학 생리학교실) ;
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Published : 2003.04.01

Abstract

The primary mechanism of smooth muscle contraction is phosphorylation of the 20 kDa myosin light chains(LC20) by a myosin light chain kinase(MLCK). Relaxation, then, is generally the result of dephosphorylation of LC20 by myosin phosphatase(MP). Changes in MP activity is one of the important mechanisms in the regulation of Ca2+-sensitivity. Inhibition of MP activity is linked to an increase in phosphorylated myosin light chain(MLC) without an increase in [Ca/sup 2+/]i-levels. It is now generally accepted that Rho-kinase phosphorylates 130 kDa regulatory and myosin binding subunits(M130, MYPT) of MP, which results in an inhibition of MP activity. In addition Rho-kinase can also directly phosphorylate MLC. In the present study, LC20 phosphorylation and MP subunits translocation to the cell membrane were investigated in freshly isolated ferret portal vein smooth muscle single cells treated with PGF2α. We also examined the effect of Y27632(10-5mol/L), Rho-kinase inhibitor, in the MP subunits localization to compare with butanol fraction of Fructus Crataegi in its effect. Butanol fraction of Fructus Crataegi(BFFC; 1㎎/㎖) was more effective in PGF2α induced contraction than those of phenylephrine in its vasodilation effect. It significantly(P<0.05) dephosphorylated the LC20 at time indicated. In addition, the dissociation of subunits are inhibited by BFCF treatment. The results indicate that, in the smooth muscle cells, the relaxation effect of BFFC is associated with increase of MP activity based on inhibition of dissociation of the catalytic and targeting subunits of the phosphatase, and thus decrease the sensitivity of LC20 phosphorylation for Ca/sup 2+/.

Keywords

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