Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effects of Guaruhaebaekbaekju-tang Extract in XO/HX-treated Rat Myocardial Cells

XO/HX에 의하여 손상된 심근세포에 대한 과루해백백주탕 추출물의 방어효과

  • Park Jun Su (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Moon Hyoung Chul (Departmental Acupuncture and Moxibustion, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Su (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kang Gil Seong (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Gyu (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Seob (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 박준수 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 문형철 (원광대학교 한의과대학 침구학교실) ;
  • 김인수 (원광대학교 한의과대학 생리학교실) ;
  • 강길성 (원광대학교 한의과대학 생리학교실) ;
  • 김인규 (원광대학교 한의과대학 생리학교실) ;
  • 김인섭 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2003.04.01
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Abstract

To certify the protective effect of herbal medicine on myocardial damage against oxygen free radical-induced myocardiotoxicity, cytotoxicity was measured using by MTT assay, LDH activity and thiobarbituric acid reactive substances(TBARS) assay in the presence of Guaruhaebaekbaekju-tang(GHBT) extracts or single constituents of this prescription, Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. In the present study, xanthine oxidase/hypoxanthine (XO/HX) resulted in a decrease in cell viability, an increase in LDH activity in culture medium and lipid peroxidation in cultured myocardial cells, In the effect of GHBT extract, it showed the prevention from the XO/HX-induced cardiotoxicity such as the decrease of LDH activity and lipid peroxidation. In the protective effect of Fructus Trichosanthis (FT) and Bulbus Allii Macrostemi (BAM), all the extracts were significantly effective in the protection of XO/HX-induced cardiotoxocity in cultured myocardial cells. From these results, they show that XO/HX is cardiotoxic in cultured myocardial cells derived from neonatal rats, and it suggests that GHBT, FT and BAM extracts are positively effective in the blocking XO/HX-induced cardiotoxicity.

Keywords

References

  1. 譯漢方醫藥學 金一赫;趙弼衡
  2. 申氏本草學 申佶求
  3. 中藥大辭典 김창민;신민교;안덕균;이경순
  4. 臨床本草學 辛民敎
  5. 漢藥臨床應用 孝尙仁;安德均;辛民敎
  6. J. Neurochem. v.51 Excitatory amino arid release from rat hippocampal slices as a consequence of free-radical formation. Pellegrini-Ciampietro D. E.;Cherici C.;Aleslani M.;Carla V.;Morroni F. https://doi.org/10.1111/j.1471-4159.1988.tb01187.x
  7. J. Neurosic. v.10 Excitatory amino acid and free radical formation may cooperate in the genesis of ichemia-induced neuronal damage. Pellegrini-Ciampietro D. E.;Cherici C.;Aleslani M.;Carla V.;Morroni F. https://doi.org/10.1523/JNEUROSCI.10-03-01035.1990
  8. J. Physiol. v.394 Permeation and block of N-methyl-D-aspartic acid receptor channels by divalent cations in mouse cultured central neurons. Mayer M. L.;Westbrook C. L. https://doi.org/10.1113/jphysiol.1987.sp016883
  9. Neuropathol, Appl. Neurobiol. v.20 Excitatory amino acid, free radicals and yhe pathogenesis of motor neuron disease Zeman S.;Lioyd C.;Meldrum S.;Leigh P. N. https://doi.org/10.1111/j.1365-2990.1994.tb00963.x
  10. J. Biol.Chem. v.245 Quantitative aspects of the production of super oxide anion radical by milk xanthine oxidase. Fridovich I.
  11. J. Biol. Chem. v.252 Liposome oxidation and erythrocyte lysis by enzymatically generated super oxide and hydrogen peroxide. Killogg E. W.;Fridovich I.
  12. Proc. Roy. Sor. London A. v.147 The catalytic decomposition of hydrogen peroxide by anion salts. Harbor F.;Weiss J.
  13. J. Biol. Chem. v.259 Iron-catalyzed hydroxyl radical formation: Stringent requirement for free iron coordination site. Craf E.;Mahoney J. R.;Bryant R. G.;Eaton J. W.
  14. Oxygen free radical involvement in ischemia and reperfusion injury to brain. v.88 Cao W.;Carney J. M.;Duchon A.;Floyd R. A.;Chevion M.
  15. 대한동의생리병리학회지 v.15 no.4 朱笑散 전탕액이 XO/HX에 의해 손상된 배양 심근세포 박동수에 미치는 영향 한동훈;권강범;황인진;김우경;김희찬;오광수;이효승;류도곤
  16. 대한동의생리병리학회지 v.15 no.4 丹參飮 전탕액이 산소자유기에 의해 손상된 심근세포의 LDH 활성도에 미치는 영향 손창식;권강범;정종선;황인진;김우경;김희란;오광수;이호승;류도곤
  17. 대한동의생리병리학회지 v.15 no.5 甘豆湯 및 加味甘豆湯 전탕액이 배양 심근세포의 LDH 활성도에 미치는 영향 박준배;권강범;김우경;오광수;김인규;이호섭;기영운;금경수;류도곤
  18. 대한동의생리병리학회지 v.15 no.5 失笑散 전탕액과 구성약물이 배양 심근세포의 LDH 활성도에 미치는 영향 한동훈;권강범;김우경;오광수;김인규;류도곤
  19. 대한동의생리병리학회지 v.16 no.2 瓜蔞薤白半夏湯 추출물이 배양 심근세포의 박동수와 LDH 활성도에 미치는 영향 안호창;권강범;박은영;장승호;류도곤
  20. 대한동의생리병리학회지 v.16 no.2 手拈散 전탕액이 배양심근세포에 미치는 영향 전영석;권강범;박은영;성은경;박승택;류도곤
  21. 대한동의생리병리학회지 v.15 no.2 丹參散전탕액이 心筋細胞 박동수에 미치는 영향 손창식;권강범;김상범;이호승;이호섭;서은아;류도곤
  22. 대한동의생리병리학회지 v.15 no.3 甘豆湯 및 加味甘豆湯 전탕액이 배양 심근세포의 총단백질량에 미치는 영향 박준배;권강범;이호승;김희찬;김우경;오광수;류도곤
  23. J. Immunol. Methods v.65 Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxirity assay. Mosmann, T. https://doi.org/10.1016/0022-1759(83)90303-4
  24. Chem. Pharm. Bull. v.35 no.1 Effort of adriamycin on cultured mouse embryomyocardial cells. K. Takahashi;Y Fujita;T. Mayumi;T. Hama;T. Kishi https://doi.org/10.1248/cpb.35.326
  25. Methods in enzymology v.52 Microsomal lipid peroxidatlon. Buese J. A.;Aust S. D.
  26. J. of Ethmophamacology v.56 Sulphorhodamine B assay for measuring proliferation melanocyte cell line and Its application to the evaluation of crude drugs used in the treatment of vililigo. Zhi Xiu Lin;J.R.S. Hoult;Amala Raman
  27. (正統)금궤요략 醫學硏究社(譯編)
  28. 금궤요략 李克光(主編)
  29. (國譯)금궤요략 李東建(編著)
  30. 方劑學 李尙仁;金東傑;李談鐘;盧昇鉉;朱榮童(共編譯)
  31. 금궤요략 再先德(主編)
  32. 금궤요략湯證論治 李文端(主編)
  33. 새漢方處方解說 具本泓
  34. Int. J. Neurosci v.57 Oxygen free radicals and brain dysfunction. Jesberger J. A.;Richardson J. S. https://doi.org/10.3109/00207459109150342
  35. J Celeb Bood Flow Metab v.13 Basic FGF, NCF, and IGFs protect hippocampal and cortical neurons against iron-induced degeneration. Zhang Y.;Tatsuno T.;Camey J. M.;Mattson M. P. https://doi.org/10.1038/jcbfm.1993.51
  36. FASEB J. v.4 Role of oxygen free radicals in carcinogenecsis and brain ischemia. Floyd R. A. https://doi.org/10.1096/fasebj.4.9.2189775
  37. 東醫心系內科學 具本泓;李京變;表亨變;金永錫;李源哲(共編著)
  38. 東醫心系內科學(上) 全國韓醫科大學 心系內科學敎室(編)
  39. Free Radical Biology & Medicine v.24 no.1 Both hydroxylamine and nitroxide protect cardiomyorytes form oxidative stress. Zhang R.;Pinson A.;Samuni A. https://doi.org/10.1016/S0891-5849(97)00165-2
  40. Toxi. Appl. Pharmacol. v.75 In vitro cadmium-DNA interactions: Cooperativity of binding and competitive antagonism by calcium, magnesium and zinc. Waalkes M. P.;Pvirier L. A. https://doi.org/10.1016/0041-008X(84)90190-X