Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Aconiti Radix on Cultured Cerebral Neurons Damaged by Reactive Oxygen Species

활성산소로 손상된 대뇌신경세포에 대한 천오두의 영향

  • Shim Jae Han (Department of Agriculture and Life Science Chonnam National University) ;
  • Lee Eun Mi (School of Medicine, Wonkwang University) ;
  • Lee Joung Hwa (School of Medicine, Wonkwang University) ;
  • Kim Dae Geun (Department of Oriental Medicine, Wonkwang University) ;
  • Lee Young Chan (Department of Oriental Medicine, Wonkwang University) ;
  • Kang Jeong Ho (Department of Oriental Medicine, Wonkwang University) ;
  • Park Sin Kee (Department of Oriental Medicine, Wonkwang University)
  • Published : 2003.04.01
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Abstract

Neurotoxicity of reactive oxygen species(ROS) and neuroprotective effect of Aconiti Radix(AR) against ROS-induced cytotoxicity were determined on cultured mouse cerebral neurons by MTT assay after cerebral neurons were cultured for 5 hours in various concentrations of GO. GO was toxic in a dose-dependent manner on cultured cerebral neurons after cerebral neurons were incubated for 5 hours in media containing 5～40mU/ml GO. While, cultures were pretreated with 180 μg/ml AR for 2 hours increased remarkably cell viability. From these results, it is suggested that GO has toxic effect on cultured mouse cerebral neurons by the decrease of cell viability. And also, herb extract such as AKR is very effective in the protection pf neurotoxicity induced by GO.

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