Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory effect of EtOAc Subfraction-2 of Spatholobus Suberectus Dunn. on tumor Metastasis

계혈등 EtOAc subfraction-2가 종양전이 억제효과에 관한 연구

  • Lee Hyun Chul (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Huh Jeong Eun (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee Jong Soo (Daewoong Pharmaceutical Co.) ;
  • Ryu Shi Yong (Korea Research Institute of Chemical technology) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 이현철 (경희대학교 동서의학대학원 종양학실험실) ;
  • 허정은 (경희대학교 동서의학대학원 종양학실험실) ;
  • 이종수 (대웅제약) ;
  • 유시용 (한국화학연구소) ;
  • 김성훈 (경희대학교 동서의학대학원 종양학실험실)
  • Published : 2003.04.01
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Abstract

Spatholobus Suberectus dunn. has been applied to blood stasis in oriental medicine. We selected one potent ethyl acetate subfraction-2 from Spatholobus Suberectus Dunn.(SSD) from anti-metastasis screening. It exerted the cytotoxicity against HT1080 and B16BL6 with the IC50 of 60 ug/ml and also significantly inhibited tumor cell induced platelet aggregation (TCIPA). It effectively didn't inhibit cell adhesion of HT1080 to matrigel coated wells, while it inhibited the cell invasion of HT1080 at the doses of 10, 20, 40 μg/ml by Boyden chamber assay. It effectively suppressed lung metastasis by B16BL6 melanoma in C57BL6 mice. These results indicate that the EtOAc subfraction-2 of Spatholobus Suberectus Dunn. can be applied to caner treatment with anti-metastatic activity.

Keywords

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