Antibacterial Activity of Curcuma longa against Methicillin-resistant Staphylococcus aureus

  • You Yong Ouk (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Yu Hyeon Hee (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeon Byung Hun (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeong Seung Il (Department of Oral Biochemistry, Department of Oral Microbiolozzzzgy, School of Dentistry, Wonkwang University) ;
  • Cha Jung Dan (Department of Oral Biochemistry, Department of Oral Microbiolozzzzgy, School of Dentistry, Wonkwang University) ;
  • Kim Shin Moo (Department of Clinical Pathology, Wonkwang Health Science College) ;
  • Kim Kang Ju (Department of Oral Biochemistry, Department of Oral Microbiolozzzzgy, School of Dentistry, Wonkwang University)
  • Published : 2003.04.01

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has been emerging worldwide as one of the most important hospital and community pathogens. Therefore, new agents are needed to treat the MRSA. In the present study, we investigated antimicrobial activity of ethyl acetate, methanol, and water extracts of Curcuma longa L. (C. longa) aganist clinical isolates of MRSA. The ethyl acetate extract of C. long a demonstrated a higher antibacterial activity than the methanol extract or water extract. Since the ethyl acetate extract was more active than other extracts, we examined whether ethyl acetate extract may restore the antibacterial activity of β-lactams and alter the adhesion and invasion of MRSA to human mucosal fibroblasts (HMFs). In the checkerboard test, ethyl acetate extract of C. longa markedly lowered the MICs of ampicillin and oxacillin against MRSA. In the bacterial adhesion and invasion assay, MRSA intracellular invasion were notably decreased in the presence of 0.125 - 2 mg/ml of C. longa extract compared to the control group. These results suggest that ethyl acetate extract of C. longa may have antibacterial activity and the potential to restore the effectiveness of β-lactams against MRSA, and inhibit the MRSA adhesion and invasion to HMFs.

Keywords

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