Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Cervi Parvum Cornu on the Cell Cycle Regulation in Human Periodontal Ligament Cells

녹용이 치주인대세포의 세포주기조절에 미치는 영향

  • You Seung Han (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Choi Hee In (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim Hyun A (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim Yun Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin Hyung Shik (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You Hyung Keun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 유승한 (원광대학교 치과대학 치주과학교실) ;
  • 최희인 (원광대학교 치과대학 치주과학교실) ;
  • 김현아 (원광대학교 치과대학 치주과학교실) ;
  • 김윤상 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실)
  • Published : 2003.02.01
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Abstract

Cervi Parvum Cornu(CPC) is that the young horn of deer family and has been traditionally used as a medicine in Eastern. The purpose of present study was to investigate the effects of CPC on cell cycle progression and its molecular mechanism in human periodontal ligament cells (HPOLC). In cell proliferation assay, 1 ng/ml, 10 ng/ml, 100 ng/ml, 1 ㎍/ml and 10 ㎍/ml of CPC were used, all treatment groups increased the cell growth. Maximal cell proliferation was observed in cells exposed to 100 ng/ml of CPC at 4 day, and 10 ng/ml and 100 ng/ml of CPC at 6 days. S phase was increased and G1 phase was decreased in the group treated with 100 ng/ml of CPC in cell cycle analysis. The protein levels of cyclin D1 were not changed, but the levels of cyclin E, cdk 2, cdk 4 and cdk 6 were increased. The protein levels of p21, pRb were decreased as compared to that of control group, but the levels of p53 was not changed in the cells both treated with CPC Md untreated. These results suggested that CPC increases the cell proliferation and cell cycle progression in HPDLC, which is linked to an increased cellular levels of cyclin E, cdk 2, cdk 4 and cdk 6, and decreased the levels of p53, p21.

Keywords

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