Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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Changes of DNA fragmentation by Irradiation Doses and Storage in Gamma-irradiated Meats and Poultry

감마선 조사 육류, 가금류에서 저장전과 후의 조사선량에 따른 DNA fragmentation의 변화

  • Lee, Hye-Jin (Department of food and Nutrition, Hannam University) ;
  • Kim, Sang-Mi (Department of food and Nutrition, Hannam University) ;
  • Park, Yoo-Kyoung (Department of food and Nutrition, Hannam University) ;
  • Yang, Jae-Seung (Laboratory for Detection of Irradiatied Foods, Korea Atomic Energy Research Institute) ;
  • Kang, Myung-Hee (Department of food and Nutrition, Hannam University)
  • 이혜진 (한남대학교 식품영양학과) ;
  • 김상미 (한남대학교 식품영양학과) ;
  • 박유경 (한남대학교 식품영양학과) ;
  • 양재승 (한국원자력연구소 식품검지실) ;
  • 강명희 (한남대학교 식품영양학과)
  • Published : 2004.04.30
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Abstract

The changes in DNA damage were investigated during storage after irradiation. Beef, pork and chicken were irradiated at 1.0, 3.0 and 5.0 kGy and stored for 6 months at $-20^{\circ}C$. The comet assay was applied to the sample muscles at the beginning of irradiation and at the end of storage. Muscles were isolated, sliced, and the suspended cells were embedded in an agarose layer. After lysis of the cells, they were electrophoresed for 2 min. and then stained. DNA fragmentation in tissues caused by irradiation was quantified as tail length and tail moment (tail length ${\times}$ % DNA in tail) by comet image analyzing system. Right after irradiation, the differences in tail length between unirradiated and irradiated muscles were significant(p<0.05) in beef, pork and chicken. With increasing the increasing doses, statistically significant longer extension of the DNA from the nucleus toward anode was observed. Similarly even 6 months after irradiation, all the irradiated muscles significantly showed longer tail length than the unirradiated controls. The results represented as tail moment showed similar tendency to those of tail length, but the latter parameter was more sensitive than the former. These results indicate that the comet assay could be one of the simple methods of detecting irradiated muscles. Moreover, this method suggest that using comet assay, we were able to detect DNA damage differences even after 6 months after irradiation.

Keywords

References

  1. Yang JS. Detection of irradiated foods. Food Sciences and Industry. 30: 121-130, 1997
  2. Farkas J. Irradiation as a method for decontaminating food. Int, J. Food Microbiol. 44: 189-204, 1998 https://doi.org/10.1016/S0168-1605(98)00132-9
  3. WHO. Wholsomeness of Irradiated Food. Report of a Joint FAO/IAEA/WHO Expert Committee. Technical Report Series. 651. Geneva, Switzerland, 1981
  4. Loaharanu P. Food irradiation: current status and future prospects. In New Methods of Food Preservation. Gould, G.W. (ed.). Blackie Academic & Professional. Glasgow. pp 90-111, 1995
  5. Park JY, Oh KN, Kim KE, Yang JS. Detection or irradiated beef and pork by DNA cornet assay. J. Korean Soc. Food Sci. Nutr. 29: 1025-1029, 2000
  6. Nam HS, Kim KE, Yang JS, Ly SY. Food majoring college students
  7. Lee CH. Acceptance and trading on irradiated foods. Korea university Press, 1998
  8. Jang HH, Kang MH, Yang JS, Ly SY. Plasma, tissue thiobarbituric acid reactive substance and lymphocyte oxidative DNA damage in mouse fed gamma irradiated diet. Korean J Nutr. 36: 255-261, 2003
  9. Tappel AL. Lipid peroxidation damage to cell to cell components. Fed Proc. 32:1870-1874, 1972
  10. Thayer OW, Christopher JP, Campbell LA. Toxicology studies of irradiation -sterilized chicken. J. Food Prot 50: 278-284, 1987 https://doi.org/10.4315/0362-028X-50.4.278
  11. Hwang KT, Park JY, Kim CK. Application of hydrocarbons as markers for detecting postirradiation of imported meats and fish. J Korean Soc Food Sci. Nutr. 26: 1109-1151, 1997
  12. Lee EY, Jung JY, Jo OJ, Kwon JH. Detection characteristics of TL, ESR and DNA cornet for irradiated peanuts by origins. J Korean Soc. Food Sci. Nutr. 30: 1076-1081, 2001
  13. Cerda H. Detection of irradiated fresh chicken, pork and fish using the DNA comet irradiated peanuts by origins. J Korean Soc. Food Sci. Nutr. 30: 1076-1081, 2001
  14. Fairbairn DW, Olive PL, O https://doi.org/10.1016/0165-1110(94)00013-3
  15. Cerda H, Delincee H, Haine H, Rupp H. The DNA 'Comet assay' as a rapid screening technique to control irradiated food. Mutat. Res. 375: 167-181, 1997 https://doi.org/10.1016/S0027-5107(97)00012-2
  16. Delincee H. DNA 'Comet assay' for rapid detection or irradiated food. Acta. Aliment. 25: 319-321, 1996
  17. Koppen G, Cerda H. Identification of low dose irradiated seeds using the neutral comet assay. Lebensm. Wiss.u. Technol. 30: 452-457, 1997 https://doi.org/10.1006/fstl.1996.0205
  18. Cerda H. Detection ot irradiated frozen food with the DNA comet assay: Interlaboratory test. J. Sci. Food. Agric. 76: 435-442, 1998 https://doi.org/10.1002/(SICI)1097-0010(199803)76:3<435::AID-JSFA969>3.0.CO;2-X
  19. Kim CK, Yang JS, Lee HJ. Detection of irradiated grains using the DNA 'Comet assay'. Korean J. Food Sci. Technol. 31: 906-911, 1999
  20. Oh KN, Park JY, Kim KE, Yang JS. Detection of irradiated fruits using the DNA comet assay. Korean J. Food Sci. Technol. 32: 531-537,2000
  21. Oh Y..N, Kim KE, Yang JS. Detection of irradiated beans using the DNA comet assay. J. Korean Soc. Food Sci. Nutr. 29: 843-848, 2000
  22. Kim SM, Park EJ, Yang JS, Kang MH. Changes of DNA fragementation by irradiation dosees and storage in gamma-irradiated fruits. J. Korean Soc. Food Sci. Nutr. 31:594-598, 2002 https://doi.org/10.3746/jkfn.2002.31.4.594
  23. Jeong SK, Park JH, Ji ST, Park KU, Kim HH, Hyun CK, Shin HK. Discrimination of irradiated beef using comet assay. Korean J. Food Sci. Technol. 32: 747-754, 2000
  24. Maerker G. Safety and Wholesomness of Irradiation Foods. International Symposium on Safety and Commercialization of Irradiated foods. pp23-29, 1994
  25. Safety evaluation of 35 kinds of irradiated human foods. Shanghai Institute of Radiation Medicine, School of Public Health, Shanghai Medical University. Chin Moo J. 100: 715-718, 1987
  26. Basson R Recent advances in radiation chemistry of vitamins. In: Elias P, Cohen A, eds. Recent advances in Food irradiation. Amsterdam, The Netherlands: Elsevier Press, 1983
  27. Diel JF. Food irradiation: Is it an alternative to chemical preservatives? Food Addit. and Contam. 9:409-416, 1992 https://doi.org/10.1080/02652039209374092