Asian-Australasian Journal of Animal Sciences

  • 제17권10호
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  • Pages.1378-1382
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  • 2004
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effects of Zinc on Lipogenesis of Bovine Intramuscular Adipocytes

  • Oh, Young Sook (Institute of Biotechnology, Yeungnam University) ;
  • Choi, Chang Bon (Department of Animal Science, Yeungnam University)
  • 투고 : 2004.01.26
  • 심사 : 2004.06.16
  • 발행 : 2004.10.01
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초록

Zinc (Zn) is a micromineral and functions as a cofactor of many enzymes and its deficiency induces retardation of growth and dysfunction of the immune system in animals. This study was conducted to determine lipogenic activity of Zn in bovine intramuscular adipocytes. Preadipocytes were isolated from intramuscular fat depots of 26 month old Korean (Hanwoo) steers and cultured in media containing Zn. At confluence, the cells were treated with insulin, dexamethasone, and 1-methyl-3-isobutyl-xanthine to induce differentiation (accumulation of lipid droplets in cells). The sources of Zn were zinc chloride (${ZnCl}_2$) and zinc sulfate (${ZnSO}_4$), and the final concentrations of both Zn sources were 0, 5, 25, 50 and 100 ${\mu}$M. Glycerol-3-phosphate dehydrogenase (GPDH) activity, an index of adipocyte differentiation, was increased as the concentration of Zn in media increased showing the highest activity (25.74 ng/min/mg protein) at 25 ${\mu}$M of ${ZnSO}_4$. Supplementation of Zn during differentiation of bovine intramuscular adipocytes tended to decrease the production of nitric oxide (NO). Peroxisome proliferator-activated receptor gamma 2(PPAR$\gamma$2) gene expression was increased 10 days after differentiation induction. The current results indicate that Zn has a strong lipogenic activity in cultured bovine intramuscular adipocytes with remarkable suppression of NO production.

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참고문헌

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  3. Effect of Rare Earth Elements on Proliferation and Fatty Acids Accumulation of 3T3-L1 Cells vol.19, pp.1, 2006, https://doi.org/10.5713/ajas.2006.119
  4. Zinc Methionine Supplementation Impacts Gene and Protein Expression in Calf-Fed Holstein Steers with Minimal Impact on Feedlot Performance vol.171, pp.None, 2004, https://doi.org/10.1007/s12011-015-0521-2
  5. Growth performance parameters, carcass traits, and meat quality of lambs supplemented with zinc methionine and (or) zinc oxide in feedlot system vol.99, pp.3, 2004, https://doi.org/10.1139/cjas-2018-0153