동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제18권6호
  • /
  • Pages.1602-1607
  • /
  • 2004
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

개채에서 분리한 3-O--D-galactopyranosylglyceride의 혈관형성 저해효과

Antiangiogenic Effect of 3--O-D-galactopyranosylglyceride Isolated from Chrysanthemum Coronarium L.

  • 이현철 (경희대학교 동서의학대학원 종양연구팀) ;
  • 송호철 (경희대학교 동서의학대학원 종양연구팀) ;
  • 임진기 (경희대학교 동서의학대학원 종양연구팀) ;
  • 길재호 (경희대학교 스포츠의학과) ;
  • 김성훈 (경희대학교 동서의학대학원 종양연구팀)
  • Lee Hyun Cheol (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Song Ho Chul (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lim Jin Ki (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Khil Jae Ho (Department of Sports Medicine, College of Sports Science, Kyunghee University) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 발행 : 2004.12.01
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초록

3-O-D-galactopyranosylglyceride (GPG; fatty acids R1, R2 = myristic acid 11.62%, palmitic acid 61.90% and oleic acid 26.48%) was isolated from Chrysanthemum coronarium L that has been used for treating renal and cardiovascular diseases as one of vegetables or medicinal drug. However, little was known about the anti-angiogenic activity of GPG. Thus, anti-angiogenic effect of GPG was evaluated in human umbilical vein endothelial cells (HUVECs) in vitro and in vivo. GPG effectively inhibited bFGF-induced migration and invasion of HUVECs in a concentration-dependent manner, whereas it did not inhibit bFGF-induced proliferation and capillary-like tube formation of HUVECs. To examine the mechanism of anti-angiogenic activity of GPG, gelatin zymography was carried out. GPG downregulated the expression of matrix metalloproteinase-2 in a concentration-dependent manner. Furthermore, GPG significantly disrupted bFGF-induced neovascularization on the chick chorioallantoic membrane assay in vivo. These results suggest that 3-O--D-galactopyranosylglyceride may inhibit neovascularization by inhibiting angiogenic activity of endothelial cells via regulation of matrix metalloproteinase-2 (MMP-2).

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