Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Angiogenic Inhibitory Effect of Zingiberis Rhizoma

건강의 혈관신생 억제효과에 관한 연구

  • Nam Sang Choon (Department of Oriental Medicine, Kyunghee University) ;
  • Myung Eu gene (Department of Oriental Medicine, Kyunghee University) ;
  • Kang Hee (Department of Oriental Medicine, Kyunghee University) ;
  • Shim Bum Sang (Department of Oriental Medicine, Kyunghee University) ;
  • Kim Sung Hoon (Department of Oncology, Kyunghee University) ;
  • Choi Seung Hoon (Department of Oriental Medicine, Kyunghee University) ;
  • Ahn Kyoo Seok (Department of Oriental Medicine, Kyunghee University)
  • 남상춘 (경희대학교 한의과대학 병리학 교실) ;
  • 명유진 (경희대학교 한의과대학 병리학 교실) ;
  • 강희 (경희대학교 한의과대학 병리학 교실) ;
  • 심범상 (경희대학교 한의과대학 병리학 교실) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 최승훈 (경희대학교 한의과대학 병리학 교실) ;
  • 안규석 (경희대학교 한의과대학 병리학 교실)
  • Published : 2004.12.01
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Abstract

This study was conducted to investigate angiogenic inhibitory effect of Zingiberis Rhizoma methanol extract using ECV-304 cells and HT1080 fibrosarcoma cells. The viability of ECV-304 was 30% at 50㎍/㎖ of Zingiberis extract and that of HT1080 was 30% at 100㎍/㎖. Using the BrdU incorporation assay, Zingiberis inhibited the DNA synthesis of ECV-304 and HT1080 by 70% and 50% at 200㎍/㎖. In tube formation assay, at 10㎍/㎖ of Zingiberis, tube network began to degrade and at higher doses, it was completely destroyed. Zymography demonstrated that Zingiberis extract decreased MMP-9 at 10㎍/㎖ and higher doses remarkably inhibited the expression of MMP-9. These data indicate that Zingiberis Rhizoma has angiogenic inhibitory effects and shows the possibility of future anti-metastatic drug.

Keywords

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