Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study on Apoptosis-Inducing Effects and Mechanism of Radix Paeoniae Alba Extract in DU145 Human Prostate Cancer Cell

백작약 추출물의 전립선 암세포 고사 유도 효과 및 기전 연구

  • Kwon Kang Beom (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim Eun Kyung (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim Kyoung Jong (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kang Gil Seong (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim Young Sun (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim In Kyu (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim In Seob (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim In Soo (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Lee Su Kyung (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Seo Eun A (Department of Food and Nutrition, School of Human Envioronmental Science, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, School of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 김경종 (원광대학교 한의과대학 생리학교실) ;
  • 강길성 (원광대학교 한의과대학 생리학교실) ;
  • 김영선 (원광대학교 한의과대학 생리학교실) ;
  • 김인규 (원광대학교 한의과대학 생리학교실) ;
  • 김인섭 (원광대학교 한의과대학 생리학교실) ;
  • 김인수 (원광대학교 한의과대학 생리학교실) ;
  • 이수경 (원광대학교 한의과대학 재활의학교실) ;
  • 서은아 (원광대학교 생활과학대학) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2004.12.01
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Abstract

The aim of this study was to investigate the apoptotic effect and its mechanism on Radix Paeoniae Alba Extract(RPAE) in DU145 human prostate cancer cell line. RPAE induced apoptosis in a dose-dependent manner in DU145 cells as confirmed by both discontinuous DNA fragmentation using Hoechst33342 staining and poly-(ADP-ribose) polymerase(PARP) cleavage, which are apoptotic signs. To clarify the mechanisms on RPAE-induced apoptosis, we examined the p50(NF-κB subunit), IκBα, PTEN and Par-4 protein expression using Western blotting. Treatment with RPAE resulted in the decrease of p50 expression by IκBα increase, which resulted in Par-4 increase and bcl-2 decrease in DU145 cells. These results suggest that apoptosis of DU145 cells by RPAE involved decreases of NF-κB activation and bcl-2 expression, increase of Par-4 protein expression.

Keywords

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