Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

In Vitro Inhibition of Pro-inflammatory Mediator mRNA Expression by Nephrite in Lipopolysaccharide-induced Mouse Macrophage Cells

RAW 264.7 면역세포에서 염증유발인자의 유전자 발현에 대한 연옥수와 연옥분의 억제효과

  • Yeom Mi-jung (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Choi Bo-Hee (Bokyung Nephrite Co.,) ;
  • Han Dong-Oh (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee Hye-Jung (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Shim Insop (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim Sung-Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Hahm Dae-Hyun (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University)
  • 염미정 (경희대학교 동서의학대학원 침구경락학교실) ;
  • 최보희 (㈜보경연옥) ;
  • 한동오 (경희대학교 동서의학대학원 침구경락학교실) ;
  • 이혜정 (경희대학교 동서의학대학원 침구경락학교실) ;
  • 심인섭 (경희대학교 동서의학대학원 침구경락학교실) ;
  • 김성훈 (경희대학교 동서의학대학원 종양학교실) ;
  • 함대현 (경희대학교 동서의학대학원 침구경락학교실)
  • Published : 2004.12.01
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Abstract

Nephrite has been widely used as a medicinal mineral resource to treat a numerous chronic diseases and to replenish vital essence and blood in the Korean traditional medicine. However, as of yet, there is little understanding of the pharmacological and biochemical mechanisms of its therapeutic effects as regards anti-inflammation. We therefore examined whether nephrite represses the expression of major inflammation mediators, such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), in LPS-stimulated murine macrophage cell line, RAW264.7 by using RT-PCR. The powder suspension and water extracts of nephrite significantly inhibited the mRNA expression of the mediators, despite a little toxic effects on growth of RAW 264.7 cells within the concentration range tested. These experimental results suggested that the nephrite can be utilized as a functional mineral exerting the anti-inflammation medicinal effect.

Keywords

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