Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Study of Sophorae Radix on $H_2O_2$-mediated Apoptosis and Total Protein Expression Pattern in Vascular Smooth Muscle Cells

고삼이 $H_2O_2$에 의한 대동맥 평활근세포 고사 및 전체 단백질 발현에 미치는 영향

  • Jeon In Cheol (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeong Jae Eun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Son In Hwan (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Ju Seok (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeong Seung Won (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Jang Jae Ho (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Seon U (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Byun Soon (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 전인철 (원광대학교 한의과대학 심계내과학교실) ;
  • 정재은 (원광대학교 한의과대학 심계내과학교실) ;
  • 손인환 (원광대학교 한의과대학 심계내과학교실) ;
  • 이주석 (원광대학교 한의과대학 심계내과학교실) ;
  • 정승원 (원광대학교 한의학 전문대학원 제3의학과) ;
  • 장재호 (원광대학교 한의학 전문대학원 제3의학과) ;
  • 이선우 (원광대학교 한의학 전문대학원 제3의학과) ;
  • 이인 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의학 전문대학원 제3의학과)
  • Published : 2004.12.01
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Abstract

Apoptosis of vascular smooth muscle cells(VSMCs) is essential in atherogenesis, being a factor that modulates its early progression rather than a terminal event in the course of the disease. Various stimuli, including oxide lipoproteins, altered hemodynamic stress and free radical, can induced VSMCs apoptosis in vitro. The protective effects of Sophorae Radix (SR) on apoptotic cell death induced by H₂O₂ were investigated in VSMCs. The viability of VSMCs was markedly decreased by H₂O₂. Sophorae Radix protected the H202-induced apoptotic death of VSMCs, which was characterized as nuclear fragmentation and increase of sub-G0/G1 fraction .. Sophorae Radix decreased the activation of caspase-3 like protease induced by H₂O₂ and recovered control level from H202-induced PARP, Bak, Bcl-XL and mitochondrial membrane potential. These results suggest that Sophorae Radix protected VSMCs apoptotic death induced by H₂O₂ via inactivation of caspase-3 and modulation of mitochondrial function. Also, the expression profile of proteins by using two-dimensional (2-D) gel electrophoresis was screened. Future investigations will need to explore the use of an anti atherosclerotic therapy of Sophorae Radix, which relies on inhibition of the proapoptotic activation of the vascular smooth muscle cells.

Keywords

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