Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-metastatic and Anti-angiogenic Activities of Ekong-san and Its Metabolites by Human Intestinal Bacteria

이공산의 혈관신생 및 암전이 억제효과에 관한 연구

  • Kang Chang Hee (Department of Oriental Medicine, Kyung Hee University) ;
  • Myung Eu Gene (Department of Oriental Medicine, Kyung Hee University) ;
  • Kang Hee (Department of Oriental Medicine, Kyung Hee University) ;
  • Choi Sun Mi (Korea Institute of Oriental Medicine) ;
  • Shim Bum Sang (Department of Oriental Medicine, Kyung Hee University) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Choi Seung Hoon (Department of Oriental Medicine, Kyung Hee University) ;
  • Shin Hyeun Kyoo (Korea Institute of Oriental Medicine) ;
  • Kim Dong Hyun (College of Pharmacy, Kyunghee University) ;
  • Ahn Kyoo Seok (Department of Oriental Medicine, Kyung Hee University)
  • 강창희 (경희대학교 한의과대학 병리학 교실) ;
  • 명유진 (경희대학교 한의과대학 병리학 교실) ;
  • 강희 (경희대학교 한의과대학 병리학 교실) ;
  • 최선미 (한국한의학연구원) ;
  • 심범상 (경희대학교 한의과대학 병리학 교실) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 최승훈 (경희대학교 한의과대학 병리학 교실) ;
  • 신현규 (한국한의학연구원) ;
  • 김동현 (경희대학교 약학대학) ;
  • 안규석 (경희대학교 한의과대학 병리학 교실)
  • Published : 2004.12.01
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Abstract

Ekong-san(EKS) was expected to have inhibitory effects on angiogenesis, considering the fact that its constituents such as Ginseng Radix, Glycyrrhizae Radix and Citri Pericarpium were reported to inhibit angiogenesis. Moreover, recently several metabolites transformed by the human intestinal microflora were reported to enhance effectiveness compared to their crude drugs. Based on these data, this study was designed to confirm whether the EKS metabolites (EKS-M) can significantly exert the anti-angiogenic and anti-metastatic activites. Hence, with EKS and EKS-M, viability assay, proliferation assay, in vitro tube formation assay, gelatin zymogram assay, in vitro invasion assay were carried out. EKS showed less toxicity in ECV304 and HT1080 cells than EKS-M. EKS-M inhibited the proliferation of HT1080 cells by 30% at 200㎍/㎖ and 42% at 400 ㎍/㎖ respectively. Also, EKS-M degraded the tube network at 200㎍/㎖. EKS and EKS-M inhibited the expression of MMP-9 at 200 and 400㎍/㎖ in HT1080 cells. EKS reduced the invasive activity of HT1080 cells through matrigel coated transfilter atthe concentration of 200㎍/㎖ more effectively than EKS-M. These data suggest that EKS and EKS-M has anti-angiogenic and anti-metastatic activities.

Keywords

References

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