Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Electro-stimulation on Stability of Alcohol Dehydrogenase from Horse Liver

알콜 탈수소효소의 안정도에 미치는 전기 자극의 효과

  • Lee Kang-Min (Division of Biology, College of Natural Science, Chonbuk National University) ;
  • Kim Chunl-Sang (Division of electronics, College of Engineering, Chonbuk National University) ;
  • Lee Hye-Jung (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Hahm Dae-Hyun (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park Chung-Ung (Division of Biology, College of Natural Science, Chonbuk National University)
  • 이강민 (전북대학교 자연과학대학 생물과학부) ;
  • 김철생 (전북대학교 공과대학 전자공학부) ;
  • 이혜정 (경희대학교 동서의학대학원 침구경락학교실) ;
  • 함대현 (경희대학교 동서의학대학원 침구경락학교실) ;
  • 박충웅 (전북대학교 자연과학대학 생물과학부)
  • Published : 2004.12.01
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Abstract

We investigated the activity and stability of alcohol dehydrogenase from horse liver (HLADH) under the electric stimulation. The activity and stability of alcohol dehydrogenase depended on electric output voltaqe, stimulation time, pulse duration and pulse interval, and temperature. HLADH retained about 23% of its activity in buffer but 78% in 10% trehalose solution under electric stimulation with 10V, 10min, The stabilizing of enzymes against electric stimulation by stabilizing additives showed a great potential use of enzymes in biotechnology and medical engineering fields.

Keywords

References

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