Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effects of Hwangryunhaedog-tang on Hypoxia-induced Apoptosis in H9c2 Cardiomyoblast Cells

황연해독탕이 저산소증에 의한 배양심근세포고사에 미치는 영향

  • Jeong Jae Eun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yu Bong Seon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Park Jin Yeong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeon In Cheol (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Park Sang Beom (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Dae Yong (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Min Goo (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Byun Soon (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 정재은 (원광대학교 한의과대학 심계내과학교실) ;
  • 유봉선 (원광대학교 한의과대학 심계내과학교실) ;
  • 박진영 (원광대학교 한의과대학 심계내과학교실) ;
  • 전인철 (원광대학교 한의과대학 심계내과학교실) ;
  • 박상범 (원광대학교 한의과대학 심계내과학교실) ;
  • 이대용 (원광대학교 한의학 전문대학원 제3의학과) ;
  • 이민구 (원광대학교 한의학 전문대학원 제3의학과) ;
  • 이인 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의학 전문대학원 제3의학과)
  • Published : 2004.12.01
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Abstract

The water extract of Hwangryunhaedog-tang(HRHDT} has been traditionally used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of HRHDT rescues cells from these damages. This study was designed to investigate the protective mechanisms of HRHDT on hypoxia-induced cytotoxicity in H9c2 cardiomyoblast cells. Hypoxia, markedly decreased the viability of H9c2 cells, which was characterized with apparent apoptptic features such as chromatin condensation as well as fragmentation of genomic DNA and nuclei. However, HRHDT significantly reduced hypoxia-induced cell death and apoptotic characteristics. Also, HRHDT prevented the mitochondrial dysfunction including the disruption of mitochondria membrane permeability transition (MPT) and an increase in expression of anti-apoptotic Bcl-2 proteins in hypoxia-H9c2 cells. Taken together, this study suggests that the protective effects of the water extract of HRHDT against hypoxic damages may be mediated by the modulation of Bcl-2 and Bak expression.

Keywords

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