Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Cytoprotective Effects of Radix Curcumae Aromaticae in Human Umbilical Vein Endothelial Cells

울금에 의한 혈관내피세포 보호 효과에 대한 연구

  • Seo Eun A (Department of Food and Nutrition, School of Human Envioronmental Science, Wonkwang University) ;
  • Chung Hun Taeg (Genome Research Center for Immune Disorders, School of Medicine, Wonkwang University) ;
  • Ko Kwang Hak (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beom (Department of Physiology, School of Oriental Medicine, Wonkwang University)
  • 서은아 (원광대학교 생활과학대학 식품영양학과) ;
  • 정헌택 (원광대학교 의과대학 면역유전체질환 연구센터) ;
  • 고광학 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실)
  • Published : 2004.12.01
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Abstract

In order to validate the use of Radix Curcumae Aromaticae as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effect of water-soluble extract of Radix Curcumae Aromaticae (ECA) on the expression of inducible heme oxygenase-1 (HO-1), which ha.s anti-inflammatory and cytoprotective effects stimulates, in human umbilical vein endothelial cells (HUVECs) stimulated with a high dose of pro-inflammatory tumor necrosis factor-alpha (TNF-α). The extract protected dose-dependently HUVECs against TNF-α-induced apoptosis, as measured qualitatively by a nuclear staining method using the fluoresoence DAPI and quantitatively by a flow cytometry using fluoresce-enhanced Annexin V antibody, and significantly Increased HO-1 expression, as determined by Western blotting analysis using anti-HO-1 antibody. Biockage of HO-1 activity by a pharmacological inhibitor reversed cytoprotection afforded by the extract, and treatment with carbon monoxide, one of HO-1 metabolites, resulted in cytoprotection comparable to the extract. These results suggest that ECA may have therapeutic potential in the control of endothelial disorders caused by inflammatory cytokines.

Keywords

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