Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study of Effects of Electroacupuncture on the Hippocampal Cholinergic Neuronal Activity

  • Lee Kwang Ro (Department of Neuroscience and Oriental Internal medicine, Gwang-Ju Oriental Medical Hospital, College of Oriental Medicine, Wonkwang University) ;
  • Kang Sei Young (Department of Neuroscience and Oriental Internal medicine, Gwang-Ju Oriental Medical Hospital, College of Oriental Medicine, Wonkwang University) ;
  • Yoon Ji Won (Department of Neuroscience and Oriental Internal medicine, Gwang-Ju Oriental Medical Hospital, College of Oriental Medicine, Wonkwang University) ;
  • Cho Chang Hyun (Department of Neuroscience and Oriental Internal medicine, Gwang-Ju Oriental Medical Hospital, College of Oriental Medicine, Wonkwang University) ;
  • Sung Kang Keyng (Department of Neuroscience and Oriental Internal medicine, Gwang-Ju Oriental Medical Hospital, College of Oriental Medicine, Wonkwang University)
  • Published : 2004.12.01
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Abstract

The purpose of this report is to investigate the electroacupuncture effect on the cholinergic neuronal activation in the hippocampal CA1 section. The electroacupuncture was performed on S36 of white rats and its consequencies were investigated by immunohistochemical method. Hippocampal CA1 sections of Sprague Dawley white male rats electroacupunctured on S36 at 20㎐ and 100㎐ are stained by cresyl violet to show that the values of 100㎐ and 20㎐ group increased significantly compared to sham group's one. Especially, 100㎐ group shows stronger neuronal activation compared to 20㎐ group. Induction of AChE, a neurotransmitter, in hippocampal CA1 is increased significantly in 100㎐ and 20㎐ group compared to sham group. Especially 20㎐ group shows higher AChE immunoreaction than 100㎐ does, although it wasn't significant enough. Induction of NGF(Nerve Growth Factor) in hippocampal CA1 sections was observed higher in 20㎐ and 100㎐ group than in sham group. Especially, 20㎐ group shows higher NGF immunoreaction compared to 100㎐. The facts above indicate that the eletroacupucture is effect to the cholinergic neuronal activation of hippocampus induced by focal ischemia.

Keywords

References

  1. Neurol Sci v.193 Distribution of m1 muscarinic acetylcholine receptors in the hippocampus of patients with alzheimer's disease and dementia with Lewy bodies-an immunohistochemical study Kazumasa Shiozaki;Eizo Iseki;Hiroaki Hino;Kenji Kosaka.
  2. Neurosci v.119 Cholinergic stimulation enhances long-term potentiation in the CA1 region of rat hippocampus Blitzer RD;Gil O;Landau EM.
  3. Neurosci Lett v.116 Muscarinic receptor activation facilitates the induction of long-term potentiation (LTP) in the dentate gyrus Burgard EC;Sarvey JM.
  4. Neurosci v.22 no.6 Achetylcholine in mind: a neurotransmitter correlate of consciousness? Elaine Perry;Matthew Walker;Jan Grace;Robert Perry.
  5. Brain Cogn. v.28 Acetylcholine and Hallucinations- Disease-Related Compared to Drug-Induced Alterations in Human Consciousness Perry, E.K.;Perry, R.H.
  6. Brain Research Review v.25 Cholinergic activity and amyloid precursor protein metabolism Melinda R. Roberson;Lindy E. Harrell.
  7. Memory Dysfunction: an Integration of Animal and Human Research from Preclinical and Clinical Perspectives v.444 A critical review of the role of the cholinergic system in human memory and cognition P. Davies.;D.S. Olton;E. Gamzu;S. Corkin(Eds.)
  8. Neurosci v.25 no.2 Alzheimer's disease and Down'n syndrome: roles of APP, trophic factors and ACh Ole Isacson;Hyemyung Seo;Ling Lin;David Albeck;Ann-Charlotte Granholm.
  9. Proc. Natl. Acad. Aci. U.S.A. v.96 Cognitive changes and modified processing of amyloid precursor protein in the cortical and hippocampal system after cholinergic synapse loss and muscarinic receptor activation Lin, L.(et al.)
  10. Ann. New York Acad. Sci. v.920 Cholinergic modulation of amyloid processin and dementia in animal models of alzheimer's disease Isacson, O.;Lin, L.
  11. Proc. Natl. Acad. Sci. U.S.A. v.97 Alzheimer-like neurodegeneration in aged antinerve growth factor transgenic mice Capsoni, S.(et al.)
  12. Br. Med. v.2 Correlation of cholinergic abnormalities with senile plaques and mental test scores in senile dementia Perry, E.(et al.)
  13. Comprehensive Textbok of Psychiatry Organic syndromes: demtia C.E. Wells.;H.I. Kaplan;B.J. Sadock(Eds.)
  14. Patient Care. v.25 no.18 Making sure it's really Alzheimer's D.A. Drachman;R.P. Fridland;E.B. Larson;M.E. Williams
  15. Neurobiology of aging. v.24 Acetylcholinesterase promotes beta-amyloid plaques in cerebral cortex T.Rees;P.I. Hammond;H. Soreq;S. Younkin;S. Brimijoin.
  16. Neurochem. Intl. v.28 no.5/6 Non-classical actions of cholinesterases: role in cellular differentiation Small DH;Michaelson S;Sberna G.
  17. Nat. Rev. Neurosci. v.2 Acetylcholinesterasenew roles for an old actor Soreq H;Seidman S.
  18. EMBO J. v.15 no.18 The structurefunction relationships in Drosophila neurotactin show that cholinesterasic domains may have adhesive properties Darboux I;Barthalay Y;Piovant M;Hipeau-Jacquotte R.
  19. J Neurochem. v.66 no.1 Overexpression of alternative human acetylcholinesterase forms modulates process extensions in cultured glioma cells Karpel R;Sternfeld M;Ginzberg D;Guhl E;Graessmann A;Soreq H.
  20. J Neurochem. v.69 no.4 Neurite differentiation is modulated in neuroblastoma cells engineered for altered acetycholinesterase expression Koenigsberger C;Chiappa S;Brimijoin S.
  21. J Neurosci. Res. v.63 no.2 Direct evidence for an adhesive function in the noncholinergic role of acetylcholinesterase in neurite outgrowth Sharma KV;Koenigsberger C;Brimijoin S;Bigbee JW.
  22. Neurochem. v.65 Neurotrophic factors attenuate glutamate-induced accumulation of peroxides, elevation of intracellular Ca2+concentration, and neurotoxicity and increase antioxidant enzyme activities in hippocampal neurons Mattson, M.P.(et al.)
  23. Neurosci. v.16 Systemic administration of a nerve growth factor conjugate reverses aged-related cognitive dysfunction and prevents cholinergic neuron atrophy Backman, C.(et al.)
  24. Comp. Neurol. v.387 Carrier mediated delivery of NGF: alterations in basal forebrain neurons in aged rats revealed using antibodies against low and high affinity NGF receptors Backman, C.(et al.)
  25. Neurosci. v.1 Degenerative changes in forebrain cholinergic nuclei correlate with cognitive impairments in aged rats Fischer, W.(et al.)
  26. Neurosci. Res. v.20 Rat beta-nerve growth factor sequence and site of synthesis in the adult hippocampus Whittemore, S.R.(et al.)
  27. Ann. Rev. Neurosci. v.24 Nerve growth factor signaling, neuroprotection, and neural repair Sofroniew, M.(et al.)
  28. Eur. Neurosci. v.7 Characterization of nerve growth factor(NGF) release from hippocampal neurons: evidence for a constitutive and an unconventional sodium-dependent regulated pathway Bloechl, A.;Thoenen, H.