Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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The Effect of Phospholipase $A_2$ Herbal-acupuncture on Neurotransmitters against Middle Cerebral Artery Occulsion Rats.

Phospholipase $A_2$ 약성이 뇌허혈을 유발시킨 흰쥐의 신경전달물질에 미치는 영향

  • Kim Sung Min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Jung Tae Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Leem Seong Cheol (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Seo Jeong Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Han Sang Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University)
  • 김성민 (대구한의대학교 한의과대학 침구학교실) ;
  • 정태영 (대구한의대학교 한의과대학 침구학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구학교실) ;
  • 서정철 (대구한의대학교 한의과대학 침구학교실) ;
  • 한상원 (대구한의대학교 한의과대학 침구학교실)
  • Published : 2004.10.01
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Abstract

This experimental studies were performed in order to prove the effect of Phospholipase A₂(PLA₂) Herbal-acupuncture by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). We observed the change of extracellular concentrations(μM) Of dopamine, DOPAC, HVA, HIAA, glutamate, aspartate, GABA, glysine, taurine, alanine, and tyrosine as extracted by vivo microdialysis, in the PLA₂ Herbal-acupuncture administrated rats(240-260g, Sprague-Dawley) subjected to the MCAO. The dialysates were extracted three times before the MCAO and six times after the MCAO every 20 minutes, and ana lysed by highperformance liquid chromatography(HPLC). PLA₂ Herbal-acupuncture significantly inhibited glutamate and tyrosine which are stimulant neurotransmitters at brain ischemia, and it significantly decreased glycine, GABA, taurine, and alanine which are inhibitory neurotransmitters at brain ischemia. PLA₂ Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

Keywords

References

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