Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Studies on the Anti-apoptotic Effect of the Mudanpi

목단피가 세포고사의 억제에 미치는 영향에 관한 연구

  • Kwon Duck Yun (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Bae Young Chun (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Sang Min (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yoo Kwan Seok (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Joo Jong Cheon (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Kyung Yo (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University)
  • 권덕윤 (원광대학교 한의과대학 사상체질의학과) ;
  • 배영춘 (원광대학교 한의과대학 사상체질의학과) ;
  • 이상민 (원광대학교 한의과대학 사상체질의학과) ;
  • 유관석 (원광대학교 한의과대학 사상체질의학과) ;
  • 주종천 (원광대학교 한의과대학 사상체질의학과) ;
  • 김경요 (원광대학교 한의과대학 사상체질의학과)
  • Published : 2004.08.01
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Abstract

Mudanpi (Cortex Moutan Radicis; the root cortex of Paeonia suffruticosa Andrews) is an important Chinese crude drug used in many oriental prescriptions. 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose (PGG), a major component of this crude drug, has been shown to possess potent antioxidant, anti-mutagenic and anti-proliferative effects. In this study, I examined whether PGG could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 expression and HO activity. Exposure of Neuro 2A cells to PGG (10-50μM) resulted in a concentration- and time-dependent induction of HO-1 mRNA, and protein expressions and heme oxygenase activity. PGG protected the cells from hydrogen peroxide-induced cell death. The protective effect of PGG on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. These results indicate that PGG is a potent inducer of HO-1 and HO-1 induction is responsible for the PGG-mediated cytoprotection against oxidative damage.

Keywords

References

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