Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Antitumor Effect of the Cotreatment of Gagamsibjeondaebo-tang and $As_2O_3$ in Human Lung Cancer Cell Line H-460

인간 폐암세포주 H-460 세포에서 가감십전대보탕과 $As_2O_3$의 병용처리에 의한 항종양 증진효과

  • Hur Jong Chan (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Won Jin Hee (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Dong Woung (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Han Se Hee (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Goo (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 허종찬 (원광대학교 한의과대학 비계내과학 교실) ;
  • 원진희 (원광대학교 한의과대학 비계내과학 교실) ;
  • 김동웅 (원광대학교 한의과대학 비계내과학 교실) ;
  • 한세희 (원광대학교 한의과대학 비계내과학 교실) ;
  • 문구 (원광대학교 한의과대학 비계내과학 교실)
  • Published : 2004.08.01
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Abstract

This study was designed to elucidate the synergistic cytotoxic mechanisms of the cotreatment of Gagamsibjeondaebo-tang (GSD) and AS₂O₃ in human lung cancer cell line, H-460. The combination of GSD and AS₂O₃ synergistically augmented the cytotoxicity of GSD and AS₂O₃ in H-460 cells. The nature of cytotoxicity was revealed as apoptosis which was characterized by chromatin condensation and fragmentaton in DAPI staining. The apoptotic cytotoxicity of GSD and AS₂O₃ was accompanied by the cleavage of PARP. Of note, the expression of pro-apoptotic BclXS protein was increased, but the expressions of Sax and BclXL was not affected in H-460 cells treated with GSD and AS₂O₃. In addition, antioxidant NAC completely blocked the apoptotic death of H-460 cells by GSD and AS₂O₃. In conclusion, this results suggest that the cotreatment of GSD and AS203 induces the synergistic apoptotis of human large cell lung cancer cell line, H-460 via the induction of BclXS and reactive oxygen species (ROS).

Keywords

References

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