Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Sibjeondaebo-Tang on Oxidative Stress of C6 Glial Cells

십전대보탕이 신경교세포의 산화적 손상에 미치는 영향

  • Ryu Ji Yong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yun Jong Min (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho Kwang Ho (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Moon Byung Soon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 류지용 (원광대학교 한의과대학 심계내과학교실) ;
  • 윤종민 (원광대학교 한의과대학 심계내과학교실, 원광대학교 한의학전문대학원 제3의학과) ;
  • 조광호 (원광대학교 한의학전문대학원 제3의학과) ;
  • 문병순 (원광대학교 한의과대학 심계내과학교실, 원광대학교 한의학전문대학원 제3의학과)
  • Published : 2004.08.01
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Abstract

Zinc ion has both essential and toxic effects on mammalian cells. The results demonstrated that the ability of zinc to act as an inducer of apoptosis in C6 glial cells. Incubation with 0.2 mM ZnCl₂ caused cell death that was characterized as apoptosis by internucleosomal DNA fragmentation, formation of apoptotic bodies, nuclear fragmentation and breakdown of the mitochondrial membrane potential. ZnCl₂-induced apoptosis of C6 glial cells was prevented by the addition of Sibjeondaebo-Tang and antioxidants including reduced glutathione, N-acetyl-L-cysteine. We further confirmed that ZnCl₂ decreased the intracellular levels of GSH as well as generation of H₂O₂ in C6 glial cells. In 2D-electrophoresis, computer-assisted comparative analysis of the respective silver stained spot patterns revealed 3 groups with strongly decreased intensity by ZnCl₂. Whereas, 3 groups with increasing intensity were recovered by Sibjeondaebo-Tang. These results suggest that Sibjeondaebo-Tang may function as an antioxidant against free radicals and be applicable to the treatment of brain cells against oxidative stress.

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