Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Immunohistochemistry of Paraffin-embedded Tissues by Super-signal Induction Method

슈퍼시그날 증폭 기술에 의한 파라핀 매몰조직의 면역조직화학염색

  • Yun Young Gab (Department of Skin & Beauty, Seojeong College) ;
  • Lee Jang Cheon (Department of Oriental Medical Prescription, Wonkwang University) ;
  • Jang Seon Il (Department of Skin & Beauty, Seojeong College)
  • 윤용갑 (서정대학교 피부미용과) ;
  • 이장천 (원광대학교 한의과대학 방제학교실) ;
  • 장선일 (상지대학교 한의과대학 방제학교실)
  • Published : 2004.08.01
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Abstract

The classical ABC (avidin-biotin peroxidase complex) method for immunohistochemistry in the paraffin-embedded tissues bring into being disadvantage such as low sensitivity of antigen detection and highly background. The biotinyl-tyramide conjugation recently introduced for sensitive immunohistochemistry was applied to light microscopy in paraffin-embedded pancreatic and liver tissues. The protocol consists of an indirect method in which 4-5㎛ tissue sections are reacted successively within a specific primary antibody, followed by a biotinylated secondary antibody, streptavidin-horseradich peroxidase (HRP), and then finally with biotinyl-tyramide. The labeling obtained for insulin and collagen antigen tested in pancreatic and liver tissues, respectively, was found to be highly specific with the labeling for each antigen confined to its particular cellular compartment. In this study, fish (flounder) serum was specially applied to remove nonspecific binding. Background levels and nospecific deposition of the staining were negligible. This results suggest that super-signal induction method by biotinyl-tyramide conjugate can readily applied to antigen detection of the paraffin-embedded tissues.

Keywords

References

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