Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Cytoprotective Effect of Organic Solvents Extracts of Sophorae Radix in H9c2 Cells

심근세포에서 고삼 유기용매 추출물의 항독성 효과

  • Kwon Kang Beom (Department of Physiology, Wonkwang University) ;
  • Kim Eun Kyung (Department of Physiology, Wonkwang University) ;
  • Lim Yang Eui (Department of Oriental Rehabilitation Medicine, Wonkwang University) ;
  • Song Yung Sun (Department of Oriental Rehabilitation Medicine, Wonkwang University) ;
  • Park Jong Ha (Department of Oriental Rehabilitation Medicine, Wonkwang University) ;
  • Moon Hyung Cheal (Department of Acupuncture & Moxibustion, School of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 임양의 (원광대학교 한의과대학 재활의학교실) ;
  • 송용선 (원광대학교 한의과대학 재활의학교실) ;
  • 박종하 (원광대학교 한의과대학 재활의학교실) ;
  • 문형철 (원광대학교 한의과대학 침구학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

To test the cytoprotective effect of sophorae radix (SR) against hydrogen peroxide (H₂O₂)-induced cytotoxicity, we investigated the cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in the presence of methylene chloride, n-butanol, ethyl acetate and water soluble fraction of SR water extracts in H9c2 cells. These results were obtained as followed; H₂O₂ decreased the cell viability of H9c2 cells in a dose dependent manner. Cells pretreated with SR water extracts were protected the H₂O₂-induced decrease of viability in H9c2 cells. Among organic solvents fractions of SR water extracts, ethyl acetate soluble fractions of SR protected the decrease of viability induced by H₂O₂ in H9c2 cells. These results suggest that ethyl acetate soluble fractions of SR water extracts is effective in the prevention of H₂O₂-induced cytotoxicity.

Keywords

References

  1. 中成藥分析 孟憲서
  2. 本草求眞 黃官수
  3. 中藥藥理毒理與臨床 李廣勛
  4. 實用中醫內科學 董黎明
  5. 한국생약학회지 v.29 no.1 한국산 생약으로부터 항암물질의 개발(제 6부); 금은화$\cdot$Ethyl Acetate 가용성 분획의 인체 구강유상피암종세포에 미치는 독성작용 한두석(외5인)
  6. 神農本草經 吳普
  7. 大韓韓醫學會誌 v.14 no.2 急性附子草烏中毒에서 甘豆湯과 苦蔘의 應用 李承武(外)
  8. 中藥通報 v.10 no.5 苦蔘總鹽抗實驗性心律失常作用的硏究 張寶鳳(外)
  9. 湯液本草 王好古
  10. 重修政和經史證類備急本草 唐愼微
  11. 中草藥學 上海中醫學院
  12. 本草備要解析 汪昻
  13. 本草綱目 李時珍
  14. 本草從新 吳儀洛
  15. 약초의 성분과 이용 문관심
  16. 本草蒙筌 陳嘉謨
  17. 臨床本草學 辛民敎
  18. 中華人民共和國藥典 中華人民共和國衛生部藥典委員會
  19. 新編中藥大辭典 江蘇新醫學院
  20. 申氏本草學 申佶求
  21. 漢藥臨床應用 李尙仁(外)
  22. 本草學 동의학연구소
  23. 本經疏證 鄒澍
  24. 실용동약학 차진헌
  25. 陳無擇三因方 陳言
  26. 大韓韓醫學會誌 v.20 no.1 苦蔘 煎湯液이 培養心筋細胞에 미치는 影響 金賢奎(外)
  27. 동의생리학회지 v.15 no.1 苦蔘 전탕액 분획물이 배양 심근세포에 미치는 영향 서재영;권강범;조현익;이호섭;서은아;김인숙;류도곤
  28. Exp Cell Res v.98 Properties of a clonal muscle cell line from rat heart. Kimes BW;Brandt BL.
  29. Biol Pharm Bull v.24 DPPH (1,1-diphenyl- 2-picrylhydrazyl) radical scavenging activity of flavonoids obtained from some medicinal plants. Okawa M;Kinjo J;Nohara T;Ono M.
  30. Acta. Physiol. Scand. Suppl. v.492 Free radicals as mediators of tissue injury. Maestro R. F.;Thaw H. H.;Bjo가 J.;Planker M.;Arfors K. E.