단천환이 Hydrogen Peroxide에 의한 심근세포 독성에 미치는 영향

Effects of Dancheonhwan on Hydrogen Peroxide-induced Apoptosis of H9c2 Cardiomyoblasts

  • 나영훈 (원광대학교 한의학 전문대학원) ;
  • 박상범 (원광대학교 한의학 전문대학원) ;
  • 정승원 (원광대학교 한의학 전문대학원) ;
  • 윤종민 (원광대학교 한의학 전문대학원) ;
  • 이인 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의학 전문대학원)
  • Na Yeong Hun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Bak Sang Beom (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Jeong Seung Won (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yun Jong Min (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee In (Department of Internal Medicine. College of Oriental Medicine, Wonkwang University) ;
  • Moon Byung Soon (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 발행 : 2004.06.01

초록

The water extract of Dancheonhwan (DCH) has been used to treat ischemic brain and heart damage in oriental medicine. However, little is known about the mechanism by which the water extract of DCH rescues cells from ischemic damage. Therefore, this study was designed to investigate the protective mechanisms of DCH on the H₂O₂-induced toxicity in H9c2 cardiomyoblast cells. Treatment of H₂O₂ markedly decreased the viability of H9c2 cardiomyoblast in a dose-dependent and time-dependent manner. The nature of H₂O₂-induced toxicity of H9c2 cells resulted from apoptotic death confirmed with genomic DNA fragmentation. DCH increased the viability of H₂O₂-treated H9c2 cells by about 23%, and partially suppressed the genomic DNA fragmentation and PARP cleavage. H₂O₂ also activated caspase-3 protease and -9 protease, but not both caspase-6 protease and -8 protease. H₂O₂ induced the mitochondria dysfunction, including mitochondria membrane permeability transition (MPT) and cytosolic release of cytochrome c from mitochondria, which was prevented in part by pretreatment of DCH. N-acetylcystein (NAC), a free-radical scavenger, alone increased the viability of H₂O₂-treated H9c2 cells in a dose-dependent manner. Furthermore, the combination of NAC with DCH significantly increased the viability of the H₂O₂-treated H9c2 cells in a dose-dependent manner. These data indicate that DCH has the protective effect on ROS-induced apoptosis of cadiomyoblast H9c2 cells.

키워드

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