Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Antitumor Effect of the Cotreatment of Paljintanghabhwajeoghwan and $As_2O_3$ in Human Lung Cancer Cell Line H-460

인간 폐암세포주 H-460세포에서 팔진탕합화적환과 $As_2O_3$의 병용처리에 의한 항종양 증진효과

  • Song Bong gil (Department of Internal Medicine. College of Oriental Medicine, Wonkwang University) ;
  • Won Jin Hee (Department of Internal Medicine. College of Oriental Medicine, Wonkwang University) ;
  • Kim Dong Woung (Department of Internal Medicine. College of Oriental Medicine, Wonkwang University) ;
  • Lee Jong Duk (Department of Internal Medicine. College of Oriental Medicine, Wonkwang University) ;
  • Moon Goo (Department of Internal Medicine. College of Oriental Medicine, Wonkwang University)
  • 송봉길 (원광대학교 한의과대학 비계내과학 교실) ;
  • 원진희 (원광대학교 한의과대학 비계내과학 교실) ;
  • 김동웅 (원광대학교 한의과대학 비계내과학 교실) ;
  • 이종덕 (원광대학교 한의과대학 비계내과학 교실) ;
  • 문구 (원광대학교 한의과대학 비계내과학 교실)
  • Published : 2004.06.01
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Abstract

This study was designed to elucidate the synergistic cytotoxic mechanisms of the cotreatment of Paljintanghabhwajeoghwan (Paljin) and As₂O₃ in human lung cancer cell line, H-460. The combination of Paljin and As₂O₃ synergistically augmented the cytotoxicity of Paljin and As₂O₃ in H-460 cells. The nature of cytotoxicity was revealed as apoptosis which characterized by chromatin condensation and fragmentation in DAPI staining. Mitochodrial membrane potential transition was observed in H-460 cells treated with Paljin and As₂O₃. The apoptotic cytotoxicity of Paljin and As₂O₃ was accompanied by the cleavage of PARP and ICAD. Of note, pro-apoptotic Bak protein was obviously increased. However, the expression of p53 was not affected by the cotreatment of Paljin and As₂O₃. In addition, the expression of DR5 was increased by the cotreatment of Paljin and AS203. This results suggest that the synergistic cytotoxicity of the cotreatment of Paljin and As₂O₃ might be caused by the changes of the expression levels of a lots proteins, such as PARP, ICAD, Bak, DR5, which play pivotal roles in survival or death of cells.

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References

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