Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Genetic Diversity and Discrimination of Astragalus Membranaceus Bunge and A. Membranaceus var. Mogholicus Using RAPD Markers

RAPD마커를 이용한 황기의 유전적 다양성 및 기원판별

  • Bang Kyang Hwan (Division of Ginseng & Medicinal Herbs, National Institute of Crop Science, RDA) ;
  • Huh Man Kyu (Department of Molecular Biology, Dongeui University) ;
  • Cho Jean Hyeang (Division of Ginseng & Medicinal Herbs, National Institute of Crop Science, RDA)
  • 방경환 (농촌진흥청 작물과학원 인삼약초과) ;
  • 허만규 (동의대학교 자연과학대학 분자생물학과) ;
  • 조준형 (농촌진흥청 작물과학원 인삼약초과)
  • Published : 2004.06.01
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Abstract

This study was carried out to differentiate the origins of Astragalus membranaceus Bunge and A. membranaceus Bunge var. mogholicus Nakai. To identify the variation of the RAPD patterns between domestic and foreign Astragalus species, 40 random primers were applied to ten accessions of A. membranaceus and six accessions of A. membranaceus var. mogholicus genomic DNA, respectively, Ten primers of 40 primers could be used to discriminate the origins and 33 polymorph isms among 44 scored DNA fragments (33 fragments are specific for A. membranaceus and A. membranaceus var. mogholicus) were generated using these primers, 75.0 % of which were polymorphic. Especially, three primers of ten primers, OPA17, OPA11 and OPB11, were useful to differentiate between domestic and foreign Astragalus species. RAPD data from the 10 primers were used for cluster analysis and cluster analysis of RAPD markers showed that the two groups are distinct genetically. Consequently, RAPD analysis was a useful method to discriminate between A. membranaceus and A. membranaceus var. mogholicus.

Keywords

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