Inhibitory Effect of Inflammatory Cytokines Secretion of Cheonggeumganghwa-tang in Mast cell

비만세포에서의 청금강화탕의 염증성 세포활성물질 분비 억제 효과

  • Choi Young Soo (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Goo (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Dong Woung (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Han Se Hee (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Won Jin Hee (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 최영수 (원광대학교 한의과대학 비계내과학 교실) ;
  • 문구 (원광대학교 한의과대학 비계내과학 교실) ;
  • 김동웅 (원광대학교 한의과대학 비계내과학 교실) ;
  • 한세희 (원광대학교 한의과대학 비계내과학 교실) ;
  • 원진희 (원광대학교 한의과대학 비계내과학 교실)
  • Published : 2004.06.01

Abstract

Cheonggeumganghwa-tang(CGT) has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of CGT, the author examined cytotoxicity and inflammatory cytokines secretion with human mast cell line, HMC-1. HMC-1 was stimulated with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187. CGT by itself had no effect on viability of HMC-1. The effects of CGT on the secretion of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 from HMC-1 were evaluated with enzyme-linked immunosorbent assay (ELISA). CGT (1 ㎎/㎖) inhibited PMA plus A23187 -induced TNF-α and IL-6 secretion, by 93.86 ± 2.05%, 68.69 ± 2.86%, respectively. CGT also inhibited the NF-κB (p50) expression. Taken together, these results suggest that CGT inhibit the production of inflammatory cytokines in HMC-1 cells through blockade of NF-κB activation.

Keywords

References

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