Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Studies on the Apoptosis-Inducing Effect of Ulmi Pumilae Cortex on Human Leukemia HL-60 Cells

  • Rhyu Jun Ki (Department of internal medicine, College of Oriental medicine, Wonkwang university) ;
  • Yu Bong Seon (Department of internal medicine, College of Oriental medicine, Wonkwang university) ;
  • Jeong Jae Eun (Department of internal medicine, College of Oriental medicine, Wonkwang university) ;
  • Bak Jin Yeong (Department of internal medicine, College of Oriental medicine, Wonkwang university) ;
  • Son In Hwan (Department of internal medicine, College of Oriental medicine, Wonkwang university) ;
  • Lee Ju Seok (Department of internal medicine, College of Oriental medicine, Wonkwang university) ;
  • Jeon Byeong Hun (Professional Graduate School of Oriental Medicine, Wonkwang university) ;
  • Mun Byung Soon (Department of internal medicine, College of Oriental medicine, Wonkwang university)
  • Published : 2004.06.01
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Abstract

The antiproliferative effect of the water extract of the branch and root bark of Ulmi Pumilae Cortex(WEUPC) was investigated on the p53-negative human leukemia cell line (HL-60). A dose- and time-dependent inhibition of cell growth was observed; this effect appears to be due to induction of apoptosis. Involvement of oxidative stress is indicated by a dose-dependent increase in intracellular reactive oxygen species levels. In addition. anti-apoptic effect was observed in the cells simultaneously treated with WEUPC and the anti-oxidant N-acetylcysteine. WEUPC did not affect the anti-apoptotic Bcl-2 and the pro-apoptotic Bax, whereas p21/sup WAF1/CIPl/ was enhanced in a dose- and time-dependent fashion; this effect was partially inhibited by N-acetylcysteine. The increase in p21/sup WAF1/CIPl/ was accompanied by a parallel accumulation of cells in the G1 phase of the cycle. These results suggest that the p53-independent induction of p21/sup WAF1/CIP/ and the induction of apoptosis may mediate the anti proliferative effect of WEUPC at least in this study; on the basis of this observation, WEUPC could be proposed as an useful adjunct to the treatment of p53-deficient tumors, which are often refractory to standard chemotherapy.

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