Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Radix Aconiti Extract on Cell Cycle Progression in HepG2 Human Hepatoma

HepG2 간암세포주기에 대한 부자 추출물의 효과

  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Eun Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Jeong Eun Sil (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Hwang In Jin (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Woo Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Sim Jeong Sub (Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Kang San (Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin Byung Cheul (Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Song Yong Sun (Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 정은실 (원광대학교 한의과대학 생리학교실) ;
  • 황인진 (원광대학교 한의과대학 생리학교실) ;
  • 김우경 (원광대학교 한의과대학 생리학교실) ;
  • 심정섭 (원광대학교 한의과대학 간계내과학교실) ;
  • 김강산 (원광대학교 한의과대학 간계내과학교실) ;
  • 신병철 (원광대학교 한의과대학 재활의학교실) ;
  • 송용선 (원광대학교 한의과대학 재활의학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2004.04.01
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Abstract

This study was to investigate the cell cycle arrest effect and its mechanism of Radix Aconiti (RA) extract in HepG2 human hepatoma cells. We used the Flow Cytometer to investigate the effects on cell cycle arrest in RA extract-treated HepG2 cells. And protein levels involved in cell cycle progression such as p53, p21, and p21 are detected by Western blotting method. RA extract induced cell cycle arrest as confirmed by increase of G0/G1 cell population, and the mechanisms were related with up-regulation of p53, p21, p27 protein expressin in HepG2 cells. These results suggest that RA may be a valuable agent for the therapeutic intervention of human hepatomas.

Keywords

References

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