DNA chip을 이용한 건선의 한방치료에 관한 유전체 연구

DNA chip Analysis of Psoriatic Skin during the Oriental Remedy

  • 김병수 (대전대학교 한의과대학 생리학교실) ;
  • 이상근 (대전대학교 동서생명과학연구원) ;
  • 김현웅 (충남대학교 의과대학 피부과) ;
  • 이증훈 (충남대학교 의과대학 피부과) ;
  • 임종순 (대전대학교 동서생명과학연구원) ;
  • 강정수 (대전대학교 한의과대학 생리학교실)
  • Kim Byoung Soo (Department of Physiology, College of Oriental Medicine, Daejeon University) ;
  • Lee Sang Keun (Institute of Traditional Medicine & Bioscience, Daejeon University) ;
  • Kim Hyun Woong (Department of dermatology, College of Medicine, Chungnam National University) ;
  • Lee Jeung Hoon (Department of dermatology, College of Medicine, Chungnam National University) ;
  • Lim Jong Soon (Institute of Traditional Medicine & Bioscience, Daejeon University) ;
  • Kang Jung Soo (Department of Physiology, College of Oriental Medicine, Daejeon University)
  • 발행 : 2004.04.01

초록

Psoriasis is a chronic inflammatory disease of the skin characterized by epidermal hyperplasia, dermal angiogenesis, infiltration of activated T cells, and increased cytokine levels, and affects 1-3% of the world-wide population. Although many immunological and clinical reports indicate a role for the immune system in the pathogenesis of psoriasis, puzzling questions about psoriasis remain unsolved. During the several decade, immunosuppressor and PUVA treatment are ubiquitously used to psoriasis therapy. But recently, to promote terminal differentiation of keratinocytes, block either NK-Tcell or T-cell activation, and interrupting the angiogenic switch represent another therapeutic opportunity in psoriasis. To keep face with immunological therapy, the needs of newly designed prescription on the psoriasis treatments were demanded. With the object of understand the psoriasis from an orient medical point of view, patients were administrated the GY during several weeks. We investigated the changes of gene expression in involved and uninvolved skin samples during the oriental remedy. Microarray data showed several important results. First, Gene expression profiling is similar to each patient. Second, precursor proteins that organize cornified envelops are decreased at the end of remedy. But genes which related to apoptosis, G-protein signalling, and lipid metabolism are increased. Third, 68.5% of clustering genes localized on the psoriasis susceptibility locus. In our results indicated that GY influence on the keratinocytes hyperproliferation by regulating the gene, which located on the psoriasis susceptibility locus.

키워드

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