Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Down-regulation of COX-2 and hTERT Expression by Healthful Decoction Utilizing Phellinus Linteus in Human Lung Carcinoma Cells

상황을 이용한 한의학적 보건기능 개선제에 의한 인체폐암세포의 증식억제에 관한 연구

  • Park Cheol (Department of Biochemistry, College of Natural Sciences, Dongeui University) ;
  • Lee Yong Tae (Department of Physiology, College of Natural Sciences, Dongeui University) ;
  • Jeong Young Kee (Department of Microbiology, College of Natural Sciences, Dongeui University) ;
  • Choi Byung Tae (Department of Anatomy, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine) ;
  • Lee Sang Hyeon (Department of Bioscience and Biotechnology, Silla University) ;
  • Choi Yung Hyun (Department of Biochemistry, College of Natural Sciences, Dongeui University)
  • 박철 (동의대학교 한의과대학 생화학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실) ;
  • 정영기 (동의대학교 자연과학대학 미생물학과) ;
  • 최병태 (동의대학교 한의과대학 해부학교실 및 한의학연구소) ;
  • 이상현 (신라대학교 생명공학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실)
  • Published : 2004.04.01
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Abstract

The objective of the present study was to investigate the effects of aqueous extract from the healthful decoction utilizing Phellinus linteus (HDPL) on the growth of human lung carcinoma A549 cells. HDPL treatment declined the cell viability of A549 cells in a concentration-dependent manner and the anti-proliferative effects by HDPL treatment were associated with morphological changes such as membrane shrinking and cell rounding up. HDPL treatment did not affect the distribution of the cell cycle. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21WAF1/CIP1 in HDPL-treated A549 cells were remained unchanged. However, HDPL treatment inhibited the expression of cyclooxygenase-2 (COX-2) mRNA and protein in a concentration-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by HDPL treatment. Taken together, these findings suggest that HDPL-induced inhibition of human lung cancer cell proliferation is associated with the inhibition of several major growth regulatory gene products, such as COX-2 and hTERT, and HDPL may have therapeutic potential in human lung cancer.

Keywords

References

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