Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibition of Platelet Aggregation by Anti-thyroglobulin Monoclonal Antibodies

Thyroglobulin에 대한 단일클론 항체의 혈소판응집 저해 작용

  • Shon Yun Hee (Department of Pharmacology and Intractable Disease Research Center, College of Medicine, Dongguk University) ;
  • Kim Cheorl Ho (Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Jeon Byung Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Nam Kyung Soo (Department of Pharmacology and Intractable Disease Research Center, College of Medicine, Dongguk University)
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 김철호 (동국대학교 한의과대학 생화학분자생물학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터)
  • Published : 2004.04.01
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Abstract

We produced twelve monoclonal antibodies(mAbs) against thyroglobulin and characterized the bindig profiles. Among them, three mAbs(TN-1, TN-2 and TN-3) were further characterized their binding specificities. TN-2 had a potent lupus anticoagulant activity and potentiated the anticoagulant effect of venom phospholipase A₂. he anticoagulant mechanism of TN-2 was elongation of the partial thromboplastin time and binding to phosphatidylserine which may have a pivot role in blood coagulation. And TN-2 was cross-reacted with ss-DNA and ds-DNA and had a characteristic of autoantibody. These results suggest that TN-2 may provide a useful tool for studying the correlation between autoimmune thyroiditis and its therapeutic effect.

Keywords

References

  1. Eur. J. Immunol. v.34 Autoantibodies in autoimmune thyroid disease promote immune complex formation with self antigens and increase B cell and CD4+ T cell proliferation in response to self antigens. Nielsen, C.H.;Hegedus, L. and Leslie, R.G. https://doi.org/10.1002/eji.200324413
  2. Clin. Exp. Rheumatol. Autoantibodies in early arthritis: advances in diagnosis and prognostication. Hueber, W.;Utz. P.J. and Robinson, W,H.
  3. N. Engl. J. Med. v.349 Development of autoantibodies before the clinical onset of systemic lupus erythematosus. Arbuckle, M.R.;McClain, M.T.;Rubertone, M.V.;Scofield, R.H.;Dennis, G.J.;James, J.A. and Harley, J.B. https://doi.org/10.1056/NEJMoa021933
  4. Proc. Natl. Acad, Sci. v.84 Structure and function of anti-DNA autoantibodies derived from a single autoimmune mouse. Shlomchik, M.J.;A.H. Aucoin; D.S. Pisetesky and M.G. Weigert. https://doi.org/10.1073/pnas.84.24.9150
  5. Neurol. Sci. v.217 Anti-neuronal autoantibody in Hashimoto's encephalopathy: neuropathological, immunohistochemical, and biochemical analysis of two patients. Oide, T.;Tokuda, T.;Yazaki, M.;Watarai, M.;Mitsuhashi, S.;Kaneko, K.;Hashimoto, T.;Ohara, S. and Ikeda, S. https://doi.org/10.1016/j.jns.2003.08.005
  6. Ann. Rev. Med. v.39 Antiphospholipid antibodies-autoantibodies with a difference. Harris, E.N.;Asherson, R.A. and Hughes, G.R.V. https://doi.org/10.1146/annurev.me.39.020188.001401
  7. Korean J. of Life Science v.12 Production and characterization of anti- thyroglobulin monoclonal antibodies Nam, K.S.;Shon, Y.H.;Baek, S.W.;Kim, C.H.;Lim, J.K. and Hwang, C.W.
  8. Thromb. Haemostas. v.54 The dilure pospholipid APTT: a sensitive assay for verification of lupus anticoagulants. Alving, B.M.;Baldwin, P.E.;Richards, R.L. and Jackson, B.J.
  9. Korean Biochem. J. v.26 Anti-platelet function of the non-saponin fraction from Panax ginseng C.A. Mayer(Korean red ginseng) Park, H.J.;Rhee, M.H.;Park, K.M.;Nam, K.Y. and Park, K.H.
  10. J. Immunol. v.146 Human extracellular recombinant phospholipase A2 induces an inflammatory response in rabbit joints. Bomalaski, J.S.;Lawton, P. amd Browning, J.L.
  11. Thrombosis Res. v.61 Effective induction of anti-phospholipid and anticoagulant antibodies in normal mouse. Igarashi, K.;Umeda, M.;Tokida, S.;Nam, K.S. and Inoue, K. https://doi.org/10.1016/0049-3848(91)90240-W
  12. Biochim. Biophys. Acta v.1046 Production and characterization of monoclonal antibodies that specifically bind to phosphatidylcholine. Nam, K.S.;Igarashi, K.;Umeda, M. and Inoue, K. https://doi.org/10.1016/0005-2760(90)90098-I
  13. J. Immunol. v.143 Effective production of monoclonal antibodies against phosphatidylserine: stereo-specific recognition of phosphatidylserine by monoclonal antibody. Umeda, M.;Igarashi, K.;Nam, K.S. and Inoue, K.