Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Puerariae Radix on c-Fos and c-Jun Expressions in Ischemic Damaged Hippocampus of Rats

갈근이 뇌허혈 손상 흰쥐의 뇌해마 c-Fos와 c-Jun 발현에 미치는 영향

  • Jo Gyu-Chil (Department of Anatomy-Meridian, College of Oriental Medicine, Kyungwon University) ;
  • Kim Youn Sub (Department of Anatomy-Meridian, College of Oriental Medicine, Kyungwon University)
  • 조규칠 (경원대학교 한의과대학 해부경혈학교실) ;
  • 김연섭 (경원대학교 한의과대학 해부경혈학교실)
  • Published : 2004.04.01
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Abstract

Objective : This study investigated a neuroprotective effect of Puerariae Radix on cerebral ischemia. Method : The global cerebral ischemia was induced by bilateral common carotid arteries occlusion under hypotension (40mmHg) in Sprague-Dawley rats. After the treatment of Puerariae Radix extract, changes of c-Fos and c-Jun expressions, immediate early genes expressed by cerebral ischemia, in the hippocampus were observed immunohistochemically. Result: The results obtained are as follows; The significant increases of c-Fos and c-Jun expressions were observed in the hippocampus of the ischemic damaged rat brains. Then Puerariae Radix treatment demonstrated significant decreases of c-Fos and c-Jun expressions in CA1 region and dentate gyrus as compared with control group. On the upregulated c-Fos expression induced by cerebral ischemia, Puerariae Radix treatment demonstrated significant decreases of c-Fos expressions in CA1 region (P<0.01) and dentate gyrus (P<0.05) as compared to the control group, but there were not a significant changes in CA2 and CA3 regions of the hippocampus. On the upregulated c-Jun expression induced by cerebral ischemia, Puerariae Radix treatment demonstrated significant decrease of c-Jun expression in CA1 region (P<0.05) as compared to the control group, but there were not a significant changes in CA2, CA3, and dentate gyrus of the hippocampus. Conclusion : These results suggested that Puerariae Radix reveals the neuroprotective effect through the reduction of immediate early genes, c-Fos and c-Jun, induced by cerebral ischemia.

Keywords

References

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