Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effects of Radix Polygalae on Dopamine-induced Cell Death in Human SH-SY5Y Dopaminergic Neuroblastoma Cells

도파민 유도성 SH-SY5Y 세포독성에 대한 원지의 방어기전 연구

  • Lee Ji Yong (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University) ;
  • Park Jae Hyeon (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University) ;
  • Kim Kyung Yeol (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University) ;
  • Kim Tae Heon (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University) ;
  • Kang Hyung Won (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University) ;
  • Lyu Young Su (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University)
  • 이지용 (원광대학교 한의과대학 신경정신과교실) ;
  • 박재현 (원광대학교 한의과대학 신경정신과교실) ;
  • 김경렬 (원광대학교 한의과대학 신경정신과교실) ;
  • 김태헌 (원광대학교 한의과대학 신경정신과교실) ;
  • 강형원 (원광대학교 한의과대학 신경정신과교실) ;
  • 류영수 (원광대학교 한의과대학 신경정신과교실)
  • Published : 2004.04.01
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Abstract

In oriental medicine, Radix Polygalae(RP) has been to treat tremors et al. But the mechanism how to decrease tremors was not known. The purpose of this study was to investigate the effect of RP on neurodegenerative disease. We used RP to execute the study of this defense mechanism on dopamine-induced cell death in human SH-SY5Y dopaminergic neuroblastoma cells. MTT assay was used to know the cytotoxicity of dopamine and the defense mechanism. As a result of this experiment, dopamine had cytotoxicity in human SH-SY5Y cells, but when it treated with RP, the cell survival rate increased. This suppressed the cell apoptosis, activation of caspase-3 protease, production of ROS, and repair of membrane potential change. In conclusion, RP has the protective effect on dopamine-induced cell death in human SH-SY5Y dopaminergic neuroblastoma cells, so this could be an effective agent on the neurodegenerative disease like Parkinsonism.

Keywords

References

  1. J Neural Transm v.19 Neuromelanin and Parkinson's disease Marsden CD
  2. 黃帝內經素問今釋 王琦(外)
  3. 張氏醫通 장로
  4. 中醫症狀鑑別診斷學 조금탁
  5. 大韓韓醫學會誌 v.14 no.1 加味鉤藤飮의 抗痙攣作用에 관한 實驗的 硏究 金德坤
  6. 東醫神經精神科學會誌 v.8 no.1 淸心溫膽湯이 白鼠의 抗痙攣, 解熱 鎭痛, 鎭痙 및 GABA-nergic system에 미치는 影響 金在亨
  7. 慶熙大韓醫大論文集 v.11 no.1 天麻水鍼이 抗痙攣效果에 미치는 影響 朴快煥
  8. 大韓針灸學會誌 v.10 no.1 葛根水鍼의 抗痙攣, 鎭痛作用에 미치는 影響 徐美榮
  9. 釣鉤藤水鍼이 鎭痛, 抗痙攣 및 鎭靜作用에 미치는 영향 申明燮
  10. 本草學 康秉秀(외)
  11. 原色臨床本草學 辛民敎
  12. 漢藥臨床應用 李商仁(외)
  13. 遠志에 의한 腦 星狀細胞로부터 炎症性 細胞活性物質 分泌의 抑制效果에 관한 연구 黃始榮
  14. 遠志 Saponin의 利尿效果 및 中樞抑制 작용에 관한 연구 朴大圭
  15. J Neurochem v.78 no.2 Apoptotic signaling in dopamine-induced cell death: the role of oxidative stress, p38 mitogen-activated protein kinase, cytochrome c and caspases Junn E;Mouradian MM https://doi.org/10.1046/j.1471-4159.2001.00425.x
  16. Neurosci Lett v.8;320 no.3 Human alpha-synuclein over- expression increases intracellular reactive oxygen species levels and susceptibility to dopamine Junn E;Mouradian MM
  17. Nature v.371 Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE Lazebnik YA
  18. Genes Dev v.9 Mice lacking ADPRT and poly ADP-ribosylation develop normally but are susceptable to skin disease. Wang ZQ https://doi.org/10.1101/gad.9.5.509
  19. J Biol Chem v.26 Bcl-XL protects BimEL-induced Bax conformational change and cytochrome c release independent of interacting with Bax or BimEL Yamaguchi H;Wang HG
  20. Cancer Cell v.2 no.3 mediate p53-independent suppression of tumorigenesis Degenhardt K;Chen G;Lindsten T;White E; BAX and BAK https://doi.org/10.1016/S1535-6108(02)00126-5
  21. Mol Pharmacol v.54 no.6 Protective effects of the antiparkinsonian drugs talipexole and pramipexole against 1-methyl-4-phenylpyridinium-induced apoptotic death in human neuroblastoma SH-SY5Y cells Kitamura Y;Kosaka T;Kakimura JI;Matsuoka Y;Kohno Y;Nomura Y;Taniguchi T https://doi.org/10.1124/mol.54.6.1046
  22. Nucleic Acids Res v.1;30 no.17 DNA double- strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis Madigan JP;Chotkowski HL;Glaser RL
  23. J Biol Chem v.31;275 no.13 Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine 139 Rogakou EP;Nieves-Neira W;Boon C;Pommier Y;Bonner WM
  24. 實用中醫內科學 黃文東
  25. 東醫精神醫學 黃義完(外)
  26. 內科診斷學 有賀槐三
  27. 대한신경과학회지 v.9 no.1 Hemiballism 1예 송창원(外)
  28. 六科證治準繩 王肯堂
  29. 東醫寶鑑 許俊
  30. 診療要鑑 金定濟
  31. 醫學綱目 樓英
  32. 傳靑主男女科;葉天士女科 傅靑主;葉天士
  33. 內科難治病的中醫治療 劑東亮
  34. 景岳全書 張介賓
  35. 諸病源候論 巢元方
  36. 中醫臨證手冊 제당임
  37. 遠志와 石菖蒲의 單獨 및 混合投與가 白鼠의 腦損傷에 미치는 영향 康永祿
  38. : Hep G2 細胞에서 에탄올 細胞毒性의 IL-1 分泌에 있어 遠志의 抑制效果 金鍾哲