Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Induction of Cdk Inhibitor p21 and Inhibition of hTERT Expression by the Aqueous Extract of Wikyung-tang in Human Lung Carcinoma Cells

인체폐암세포의 성장에 미치는 위경장의 영향에 관한 연구

  • Choi Hae-Yun (Pohang Oriental Hospital of Daegu Haany University, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine) ;
  • Park Cheol (Department of Biochemistry, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine) ;
  • Choi Yung Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine) ;
  • Park Dong Il (Department of Internal Medicine, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine)
  • 최해윤 (대구한의대학교 포항한방병원, 동의대학교 한의과대학) ;
  • 박철 (동의대학교 한의과대학 생화학교실 및 한의학연구소) ;
  • 최영현 (동의대학교 한의과대학 생화학교실 및 한의학연구소) ;
  • 박동일 (동의대학교 한의과대학 내과학교실)
  • Published : 2004.04.01
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Abstract

In the present study, we investigated the anti-proliferative effects of aqueous extract of Wikyung-tang(WKT) on the growth of human lung carcinoma cell line A549. WKT treatment declined the cell viability and proliferation of A549 cells in a concentration-dependent manner. The anti-proliferative effects by WKT treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. WKT treatment induced an inhibition and/or degradation of apoptotic target proteins such poly(ADP-ribose) polymerase (PARP) and phospholipase C-γ1 (PLC-γ1). WKT treatment did not affect the levels of other Bcl-2 family gene products, such as Bcl-2, Bax and Bad. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 were induced by WKT treatment in A549 cells. Additionally, WKT treatment induced the down-regulation of telomerase reverse transcriptase mRNA (hTERT) expression of A549 cells, however, the levels of other telomere-regulatory gene products were not affected. Taken together, these findings suggest that WKT-induced inhibition of human lung cancer cell proliferation is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products and WKT may have therapeutic potential in human lung cancer.

Keywords

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