Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Pharmacological Effects of Bioactive Fractions from Brachyglottis monroi

  • Kwag Jung Sook (Department of Dental Hygiene, Mokpo Science College) ;
  • Na Young Soon (Division of Fashion & Beauty, Konyang University) ;
  • Perry Nigel B. (Plant Extracts Research Unit, New Zealand Institute for Crop & Food Research Ltd., Department of Chemistry, Otago University) ;
  • Kim Hyung Min (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Institute of Basic Natural Sciences, Wonkwang University) ;
  • Baek Seung Hwa (Department of Pharmacology, Kyunghee University)
  • Published : 2004.02.01
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Abstract

The effects of bioactive fractions from Brachyglottis monroi on biological activity were investigated. this bioactive subfraction 6-5 is the most cytotoxic to P388 murine leukaemia cell lines. A comparison of IC/sub 50/ values of these subfraction in cancer cell lines showed that their susceptibility to these subfractions decreased in the following order; Fr. 6-5 > Fr. 6-3 > Fr. 6-6 > Fr. 6-1 > Fr. 6-2 > Fr. 6-4 by the MTT method. Silica gel flash column chromatography concentrated the cytotoxic activity in subfraction 6-5 which eluted with 30% and 40% ethyl acetate : hexane gave a major bioactive (51 mg, P388 IC/sub 50/ 8,286 ng/mL at 7.5 ㎍/disc).

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