Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

Development of Epidermal Idioblasts in the Reproductive Structures of Lycopersicon esculentum

토마토 (Lycopersicon esculentum) 표피조직의 이형세포 분화 발달

  • 박은희 (계명대학교 자연과학대학 생물학과) ;
  • 김인선 (계명대학교 자연과학대학 생물학과)
  • Published : 2004.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Plants of Lycopersicon esculentum, containing various organic compounds, are known to develop idioblasts in their epidermis. Lycopersicon esculentum have long been investigated in many areas, but structural aspects of the epidermis of various organs have not been carried out in detail. Thus, the present study attempted to reveal the patterns of idioblast development, particularly those of the reproductive organs, in L. esculentum epidermis using scanning electron microscopy. The present study mainly focused on patterns of the stomata and trichome types. Two types of stomata were developed in the flowers and fruits: anomocytic stomata (stomata type I) were distributed normally throughout the epidermis, whereas actinocytic raised stomata (stomata type II) were found variously in different epidermal tissues. For the trichomes, both glandular and non-glandular types were developed in the epidermis. The former included peltate glandular trichomes having four head cells (trichome type I) and capitate multicellular glandular trichomes (trichome type II). The latter included non-glandular short trichomes (trichome type III) and considerably elongated trichomes with basal rosette cells (trichome type IV). In paticular, the raised stomata were well-developed in the peduncles and the peltate glandular trichomes were prominent in the sepal and ovary epidermis. Transmission electron microscopy on the ontogeny and ultrastructural differentiation of these idioblasts, associated with the current result, will aid us in better understanding of the structure and functional relationship in the epidermal differentriation of Lycopersicon esculentum.

식물체에 여러 유기성분을 함유하는 발향성 식물인 토마토는 표피조직에 여러 형태의 이형세포가 발달한다. 유용식물인 토마토에 대하여는 많은 영역에서 활발하게 집중 연구되고 있으나, 표피조직에 발달하는 이형세포에 촛점을 두고 구조적으로는 자세히 연구되어 있지 않고 있다. 이에 본 연구에서는 토마토 표피조직, 특히 생식기관의 표피조직에 발달하는 이형세포들의 구조 및 분화 발달양상을 주사전자현미경적 방법으로 연구하였다. 토마토 표피조직에는 불규칙형 기공과 방사형 돌출기공의 두 유형의 기공이 발달하였으며, 모용은 분비성과 비분비성의 다양한 4가지 형태로 분화하였다. 분비성 모용은 peltate 형과 capitate형 분비모로 대별되었고, 비분비성 모용은 단상모와 원좌형 기저세포의 장상모로 발달하였다. 특히, 화경에는 돌출기공의 발달이 현저하였고, peltate형 분비모는 꽃받침과 자방 표피조직에 뚜렷하였다. 이후 분비모용과 돌출기공에 대한 세포학적 측면에서의 구조적인 연구가 병행되어 본 결과와 접목되면 구조와 기능간의 관계규명에 많은 도움을 줄 것이며, 유용성분의 자원화 및 기능물질 개발에 있어 더욱 의미있는 연구가 될 것이다.

Keywords

References

  1. Ascensao L, Pais MS: The leaf capitate trichomes of Leonotis leonurus: histochemistry, ultrastructure and secretion. Ann Bot 81 : 263 271, 1998 https://doi.org/10.1006/anbo.1997.0550
  2. Channarayappa, Shivashankar G, Muniyappa V, Frist RH: Resistance of Lycopersicon species to Bemisia tabaci, a tomato leaf curl virus vector. Can J Bot 70 : 2184 2192, 1992 https://doi.org/10.1139/b92-270
  3. Chatterjee J, Chatterjee C: Management of phytotoxicity of cobalt in tomato by chemical measures. Plant Sci 164 : 793 801, 2003 https://doi.org/10.1016/S0168-9452(03)00066-9
  4. Cho BS, Ko KN, Kim ES: Ultrastructural study of glandular trichomes in Pelargonium peltatum. Kor J Electron Microsc 29 : 125 136, 1999
  5. Cosima B, Wiese CB, Pell EJ: Oxidative modification of the cell wall in tomato plants exposed to ozone. Plant Physiol Bioch 41 : 375 382, 2003 https://doi.org/10.1016/S0981-9428(03)00033-0
  6. Ding CK, Wang CY: The dual effect of methyl salicylate on ripening and expression of ethylene biosynthetic genes in tomato fruit. Plant 164 : 589 596, 2003 https://doi.org/10.1016/S0168-9452(03)00010-4
  7. Esau. K: Anatomy of Seed Plants. 2nd ed. Wiley. New York. pp.12 16, 1979
  8. Fahn A: Plant Anatomy. 4th ed. Pergamon Press, Oxford, pp.152 184, 1990
  9. Fahn A: Structure and function of secretory cells. In: Hallahan DL, Gray JC, Callow JA, eds, Advances in Botanical Research. Plant Trichomes, pp. 37 75, Academic Press, New York, 2000
  10. Glover BJ, Martin C: Speciation of epidermal cell morphology. In: Hallahan DL, Gray JC, Callow JA, eds, Advances in Botanical Research. Plant Trichomes, pp. 193 217, Academic Press, New York, 2000
  11. Hallahan DL, Gray JC & Callow JA: Advances in Botanical Research: Plant Trichomes. Academic Press. New York. pp.1 316, 2000
  12. Hiroaki K, Tokao K: Transgenic tomato plant carrying a gene for NADP dependent glutamate dehydrogenase from Aspergillus nidulans. Plant Sci 164 : 35 42, 2003 https://doi.org/10.1016/S0168-9452(02)00325-4
  13. Karabourniotis G, Papadopoulos K, Papamarkou M, Manetas Y: Ultraviolet B radiation absorbing capacity of leaf hairs. Physiol Plantarum 84 : 414 418, 1992
  14. Karabourniotis G, Kyparissis A, Manetas Y: Leaf hairs of Olea europaea L. protect underlying tissue against UV B radiation damage. Environ Expt Bot 33 : 341 345, 1993 https://doi.org/10.1016/0098-8472(93)90035-E
  15. Kim IS, Fisher DG: Structural aspects of the leaves of seven species of Portulaca growing in Hawaii. Can J Bot 68 : 1803 1811, 1990 https://doi.org/10.1139/b90-233
  16. Kolb D, M$\"u$ller M: Light, conventional and environmental scanning electron microscopy of the trichomes of Cucurbita pepo subsp. pepo var. styriaca and histochemistry of glandular secretory products. Ann Bot 94 : 515 526, 2004 https://doi.org/10.1093/aob/mch180
  17. Lee Y S: Modern Plant Morphology. Woosung Publishing Co., Seoul,pp. 181 204, 2000. (Korean)
  18. Luckwill LC: : An Historical, Biological, and Taxonomic Survey of the Wild and Cultivated Tomatoes. Aberdeen University Press, Aberdeen. pp. 218 224, 1943
  19. Mathew J, McCracken D: B. West Publishing. San Francisco. pp. 152 159, 1995
  20. Mauseth JD: Botany: An Introduction to Plant Biology, 2nd ed. Saunders College Publishing, Boston, pp. 167 198, 1988
  21. Mulholland BJ, Taylor IB, Jackson AC, Thompson AJ: Can ABA mediate responses of salinity stressed tomato? Environ Expt Bot 50 : 17 28, 2003 https://doi.org/10.1016/S0098-8472(02)00110-7
  22. Reeves AF: Tomato trichomes and mutations affecting their development. Amer J Bot 64 : 186 189, 1977 https://doi.org/10.2307/2442106
  23. Sekhar KNC, Sawhney VK: A scanning electron microscope study of the development and surface features of floral organs of tomato (Lycopersicon esculentum). Can J Bot 62: 2403 2413, 1984 https://doi.org/10.1139/b84-328
  24. Sohn TH, Cheon SH, Choi SW, Moon KD: Identification of flavor components in tomato fruit. J Kor Agric Chem Soc 31 : 292 297, 1988
  25. Sohn TJ: Diagnostic Electron Microscopy. Daehan Textbook Co, Seoul, pp. 83 111, 1991
  26. Snyder JC, Cater CD: Trichomes on leaves of Lycopersicon hirsutum, L. Esculentum and their hybrids. Euphytica 34 : 53 64, 1985 https://doi.org/10.1007/BF00022863
  27. Turner GW, Lersten NR: Raised stomatal clusters on Coleus (Lamiaceae) stems. Amer J Bot 70 : 975 977, 1983 https://doi.org/10.2307/2442804
  28. Valkama E, Salminen J, Koricheva J, Pihlaha K: Changes in leaf trichomes and epicuticular flavonoids during leaf development in three birch taxa. Ann Bot 94 : 233 242, 2004 https://doi.org/10.1093/aob/mch131
  29. Werker E: Trichome diversity and development. In: Hallahan DL, Gray JC, Callow JA, eds, Advances in Botanical Research. Plant Trichomes, pp. 1 35, Academic Press, New York, 2000