Healing Effects of Astragali Radix Extracts on Experimental Open Wounds in Rats

흰쥐의 외과적 창상에 대한 황기(黃耆) 추출액의 치료효과

  • Han, Dong-Oh (Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Gun-Ho (Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Choi, Yong-Bok (Bio Research Team, Central Research Institute, Hyosung Co.) ;
  • Shim, In-Sop (Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Hye-Jung (Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Yong-Gheun (Department of Oriental Pediatrics, Bundang Oriental Hospital, Dongguk University) ;
  • Kim, Jang-Hyun (Department of Oriental Pediatrics, Bundang Oriental Hospital, Dongguk University) ;
  • Chang, Gyu-Tae (Department of Oriental Pediatrics, Bundang Oriental Hospital, Dongguk University) ;
  • Hahm, Dae-Hyun (Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
  • 한동오 (경희대학교 동서의학대학원 동서의학과) ;
  • 김건호 (경희대학교 동서의학대학원 동서의학과) ;
  • 최용복 ((주)효성 중앙연구소 BIO연구팀) ;
  • 심인섭 (경희대학교 동서의학대학원 동서의학과) ;
  • 이혜정 (경희대학교 동서의학대학원 동서의학과) ;
  • 이용근 (동국대학교 분당한방병원 한방소아과학교실) ;
  • 김장현 (동국대학교 분당한방병원 한방소아과학교실) ;
  • 장규태 (동국대학교 분당한방병원 한방소아과학교실) ;
  • 함대현 (경희대학교 동서의학대학원 동서의학과)
  • Published : 2005.02.25

Abstract

Inhibition of inflammatory response, acceleration of basal cell growth, and balanced synthesis of extracellular matrix (ECM) are important in healing of cutaneous open wounds. In order to evaluate the healing effects of water extracts of Radix Astragali (the root of Astragalus membranaceus (Fisch.)) on open wound at early stage, the experimental open wounds were generated on the dorsal sides of SD rats under anesthesia. The boiled-water extracts of Radix Astragali $(100{\mu}l)$, soaked into an occlusive film dressing were applied once a day for eleven consecutive days. The healing process was assessed by measuring macroscopic appearance and wound areas of the open wounds. The molecular aspects of healing process by Radix Astragali extracts were also investigated by Hematoxylin-Eosin (H-E) double staining and immunohistological staining of collagen type I in the healed skin area, implying cell density and linear alignment of the granulation tissue, and ECM synthesis and its remodeling, respectively. The Astragali radix extracts were found to significantly accelerate the cutaneous wound healing by suppressing the inflammation and stimulating the basal cell growth in wounded area, as compared to epidermal growth factor (EGF).

Keywords

References

  1. H. J. Wang, H. L. Wan, T. S. Yang, D. S. Wang, T. M. Chenand, and D. M. Chang, Acceleration of skin graft healing by growth factors. Burns, 22(1): 10-16, 1996.
  2. Tetsuya Ishikawa, Hiroshi Terai, Takashi Yamamoto, Kazumichi Harada, Takashi Kitajima, Delivery of a growth factor fusion protein having collagen-binding activity to wound tissues. Artificial Organs. 27(2): 147-154, 2003. https://doi.org/10.1046/j.1525-1594.2003.07009.x
  3. Jane CJ Chao, Kuo-Yu Liu, Sheng-Hsuan Chen, Chia-Lang Fang, and Chili-Wei Tsao, Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer. World J Gastroenterol 9(10): 2261-2265, 2003.
  4. Tobias A. Beyer, Sabine Werner, Clive Dickson and Richard Grose, Fibroblast growth factor 22 and its potential role during skin development and repair. Experimental Cell Research, 287: 228-236, 2003. https://doi.org/10.1016/S0014-4827(03)00139-3
  5. David Chin, Glen M. Boyle, Peter G. Persons, and William B. Coman, What is transforming growth factor-beta(TGF-$\beta$)? The British Association of Plastic Surgeons 57: 215-221, 2004. https://doi.org/10.1016/j.bjps.2003.12.012
  6. Yoshiko Abe, Kouji Inagaki, Akihiko Fujiwara, and Kiyoshi Kuriyama, Wound healing acceleration of a novel transforming growth factor-beta inducer, SEK-1005, Eur J Pharmacol, 408: 213-218, 2000.
  7. William R. Tyor, Nicholas Avgeropoulos, George Ohlandt, and Edward L. Hogan, Treatment of spinal cord impact injury in the rat with transforming growth factor-beta. J Neurol Sci 200: 33-41, 2002.
  8. Hiroshi Ueno, Haruo Yamada, Ichiro Tanaka, Naoki Kaba, Mitsunobu Matsuura, Masahiro Okumura, Tsuyoshi Kadosawa, and Toru Fujinaga, Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials. 20: 1407-1414, 1999. https://doi.org/10.1016/S0142-9612(99)00046-0
  9. Buckley A, Davidson JM, and Kamerath CD, Sustained release of epidermal growth factor accelerates wound repair. Proc Natl Acad Sci. 82: 7340-7344, 2000. https://doi.org/10.1073/pnas.82.21.7340
  10. Manxi L, Donald JW, and Qin F, Relationships between nerves and myofibroblasts during cutaneous wound healing in the developing rat. Cell Tissue Res. 297: 423-433, 1999. https://doi.org/10.1007/s004410051369
  11. M.J. Muller, M.A. Hollyoak, Z. Moaveni, Tim La H. Brown, D.N. Herndon, and J.P. Heggers, Retardation of wound healing by silver sulfadiazine is reversed by aloe vera and nystatin. Burns. 29: 834-836, 2003.
  12. 鄒澍 지음. 임진석 옮김, 本經疏證 上권, 대성의학사, pp.109-114, 2001.
  13. Junge K, Rosch R, Bialasinski L, Klinge U, Klosterhalfen B, and Schumpelick V. Persistent extracellular matrix remodelling at the interface to polymers used for hernia repair. Eur Surg Res. 35(6): 497-504, 2003. https://doi.org/10.1159/000073389
  14. Sabiston's textbook of surgery (15th Ed). pp.279-303.
  15. 全國韓醫科大學 本草學敎授 共編著, 本草學, 圖書出版 永林社, pp.534-535, 1999.
  16. 田炳薰, Influence of the extract Radix Astragali on the cytotoxicity induced by the chemotherapeutic agents, mitomycion C. 동의생리병리학회지. 12(1): 55-59, 1998.
  17. 강희, 배현수, 안규석, Astragali Radix increases survival and activity of spleen lymphocytes. 동의생리병리학회지 15(4): 560-565, 2001.
  18. Kingsnorth A.N, Vowles R, and Nash J.R.G, Epidermal growth factor increases tensile strength in intestinal wounds in pig. Br. J. Surg 77: 409-412, 1990. https://doi.org/10.1002/bjs.1800770417
  19. Zhou LJ, Matsui R, and Ono I. Development of a chronic skin defect model and a study of cytokine secretion using the model. Wound Repair Regen. 8(4): 304-318, 2000. https://doi.org/10.1046/j.1524-475X.2000.00304.x
  20. Sugihara A, Sugiura K, Morita H, Ninagawa T, Tubouchi K, Tobe R, Izumiya M, Horio T, Abraham NG, Ikehara S. Promotive effects of a silk film on epidermal recovery from full-thickness skin wounds.Proc Soc Exp Biol Med. 225(1): 58-64, 2000. https://doi.org/10.1046/j.1525-1373.2000.22507.x
  21. Wang Z, Inokuchi T, Nemoto TK, Uehara M, and Baba TT. Antisense oligonucleotide against collagen-specific molecular chaperone 47-kDa heat shock protein suppresses scar formation in rat wounds. Plast Reconstr Surg, 111(6): 1980-1987, 2003. https://doi.org/10.1097/01.PRS.0000054844.41243.F2
  22. Schultze-Mosgau S, Blaese MA, Grabenbauer G, Wehrhan F, Kopp J, Amann K, Rodemann HP, and Rodel F. Smad-3 and Smad-7 expression following anti-transforming growth factor beta 1 (TGF betal)-treatment in irradiated rat tissue. Radiother Oncol. 70(3): 249-259, 2004. https://doi.org/10.1016/j.radonc.2004.01.010
  23. Yusof NL, Wee A, Lim LY, and Khor E. Flexible chitin films as potential wound-dressing materials: wound model studies. J Biomed Mater Res 66(2): 224-232, 2003.
  24. Kubo K, Kuroyanagi Y. Spongy matrix of hyaluronic acid and collagen as a cultured dermal substitute: evaluation in an animal test. J Artif Organs. 6(1): 64-70, 2003. https://doi.org/10.1007/s100470300010
  25. Romanos GE, Strub JR. Effect of Tissucol on connective tissue matrix during wound healing: an immunohistochemical study in rat skin. J Biomed Mater Res. 39(3):462-468, 1998. https://doi.org/10.1002/(SICI)1097-4636(19980305)39:3<462::AID-JBM17>3.0.CO;2-C
  26. Qiu C, Coutinho P, Frank S, Franke S, Law LY, Martin P, Green CR, and Becker DL. Targeting connexin43 expression accelerates the rate of wound repair. Curr Biol. 13(19): 1697-1703, 2003. https://doi.org/10.1016/j.cub.2003.09.007
  27. Coutinho P, Qiu C, Frank S, Tamber K, Becker D. Dynamic changes in connexin expression correlate with key events in the wound healing process. Cell Biol Int. 27(7): 525-541, 2003. https://doi.org/10.1016/S1065-6995(03)00077-5
  28. 조직학교재 편찬회. Histology: A text and Atlas, 제3판, 고문사. pp85-118, 1997.