Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidant Effects of Psoraleae Fructus in GC-1 Cells

보골지(補骨脂)가 남성 생식세포 GC-1의 항산화에 미치는 영향

  • Oh, Myung-Sook (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Do-Rim (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, So-Yeon (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Chang, Mun-Seog (Department of Medicine, Division of Newborn Medicine, Children's Hospital and Harvard Medical School) ;
  • Park, Seong-Kyu (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 오명숙 (경희대학교 한의과대학 방제학교실) ;
  • 김도림 (경희대학교 한의과대학 방제학교실) ;
  • 김소연 (경희대학교 한의과대학 방제학교실) ;
  • 장문석 (하버드대학교 의과대학 소아병원) ;
  • 박성규 (경희대학교 한의과대학 방제학교실)
  • Published : 2005.02.25
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Abstract

The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Psoraleae fructus. The extract was studied for dipheny-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being 0.427 mg/mL. The extract was dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (67.7 %) was blocked by the extract concentration- dependently. Furthermore, the extract also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Psoraleae fructus has potent antioxidant activity.

Keywords

References

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