Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Evaluation of Safety with Gamgung-tang Using Rec assay and Enzymatic Methods

Rec assay 및 효소학적 방법을 이용한 감궁탕의 안전성평가

  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Baek, Tae-Seon (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Moon, Ji-Sun (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Kim, Mee-Kyung (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Kim, Cheorl-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Medicine, Wonkwang University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 백태선 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 문지선 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 김미경 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 김철호 (동국대학교 한의과대학 생화학분자생물학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소)
  • Published : 2005.02.25
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Abstract

Gamgung-tang(GGT) that is included in Gamdu-tang(consists of Glycyrrhizae Radix, black beans) and Gunggui-tang(consists of Angelicae Radix and Cnidii Rhizoma), showed therapeutic effects of autoimmume thyroiditis in the previous reports. GGT was tested for the safety using Rec assay and enzymatic methods. In the Rec assay, Bacillus subtilis H-17$(Rec^+)$ and M-45$(Rec^-)$ strains were used to test DNA damage activity. From the results, there was no DNA damage of GGT. Hepatotoxicity of GGT to female ICR mice was also monitored by the measurements of serum(s)-GOT, s-GPT and LDH activities after oral feeding for 15 days. GGT was not shown any significant changes of s-GOT, s-GPT and LDH activities in mice sera.

Keywords

References

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