Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Ginseng Radix Extract on the Cytokine of Experimental Asthma induced by Ovalbumin

인삼(人蔘)추출물이 Ovalbumin으로 유도된 천식(喘息)의 Cytokine에 미치는 영향

  • Published : 2005.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the effect of oral administration of GSRE against the asthma. Asthma was induced to Balb/c mouse by i.p. injection and aerosol immunization with ovalbumin. It was observed the change of the eosinophil number in the BALF. Concentrations of IL-4, IL-5 in BALF and splenocyte were assessed by ELISA, IgG and IgE from serum were calculated by same method. Concentration of IL-4 in splenocyte was significantly decreased in GSRE group compared with control group. Concentrations of IL-5 from BALF and splenocyte were significantly decreased in GSRE group compared with control group, respectively. Level of IgE in serum was significantly decreased in GSRE group compared with control group, but not IgG. We found that the effect of GSRE extract in asthma was implicated in reductions of IL-4, IL-5 released from Th2 cell, and decreses of IgE, from plasma cell. These findings suggest that GSRE extract can produce anti-asthmatic effect, which may play a role in allergen-induced asthma therapy.

Keywords

References

  1. Cohn, L., Elias, J.A., Chupp, G.L. Asthma: mechanisms of disease persistence and progression. Annu Rev Immunol. 22, 789-815, 2004.
  2. 공중보건편집위원회. 예방의학과 공중보건, P 129-145, 서울, 계축문화사, 1992.
  3. Chiang, D.J., Ye, Y.L., Chen, W.L., Lee, Y.L., Hsu, N.Y., Chiang B.L. Ribavirin or CpG DNA sequence-modulated dendritic cells decrease the IgE level and airway inflammation. Am J Respir Crit Care Med. 168, 575-580, 2003.
  4. Corrigan, C.J., Haczku, A., Gemou-Engesaeth, V., Doi, S., Kikuchi, Y., Takatsu, K., Durham, S.R., Kay, A.B. CD4 T-Iymphocyte activation in asthma is accompanied by increased serum concentrations of interleukin 5. Am Rev Respir Dis. 147, 540-547, 1993.
  5. 李珩九, 鄭昇杞. 東醫肺系內科學. P 187-202, 426, 460-461, 468, 서울, 民瑞出版社， 1999.
  6. 전국의과대학교수역. 오늘의 진단 및 치료. P 287-297, 서울, 한우리, 1999.
  7. 原安微中醫學院編. 中醫臨床手冊. p 115-116, 서울, 成輔社, 1983.
  8. 上海中醫學院編. 中醫內科學. p 17-23, 香港, 商務印書館香港 分館, 1975.
  9. 李珩九. 五拗湯이 咳嗽 喘息에 미치는 영향. 경희대학교 대학원 1982.
  10. 鄭昇杞. 定喘湯이 喘息에 미치는 영향에 관한 實驗的 硏究. 경희대학교 대학원. 1985.
  11. 鄭昇杞. 加味淸上補下湯 이 喘息에 미치는 영향에 관한 實驗 的 硏究. 대한한의학회지, 12(1):118-138, 1991.
  12. 朴光恩. 千緡導痰湯이 喘息에 미치는 영향에 관한 實驗的 硏 究. 경희대학교 대학원. 1993.
  13. 이은, 고흥, 김이화, 김호현. 인삼전탕액이 신경아세포주에서 에탄올에 의해 유발된 아폼토시스에 미치는 영향. 한방내과학회지. 23(3):423-432, 2002.
  14. 서호석, 한양희, 박종형, 전찬용, 김동우, 박세기, 이청정혜, 고승희, 고재철, 최유경, 백은기,홍의실, 박지윤. 인삼이 Cyclosporin A로 유발된 백서의 신손상에 미치는 영향. 한방내과학회지. 22(1):45-54, 2001.
  15. 윤성헌, 조형래, 김동일, 이태균, 남경수. 인삼이 양수감염에 의한 조산에 미치는 영향. 부인과학회지. 13(2):149-165, 2000.
  16. 의학교육연수원. 가정의학. P 286-288, 서울, 서울대학교출판부. 1998.
  17. 馬康慈. 中醫師臨床手冊. p 235-240, 臺北, 衆文圖書公司. 1976.
  18. 貝本泓, 朴鎬湜, 尹泰汝, 金東圭, 崔治求. 東醫內科學. p 41-47. 서울, 書苑堂. 1992.
  19. Lampinen, M., Carlson, M., Hakansson, L.D., Venge, P. Cytokine-regulated accumulation of eosinophils in inflammatory disease. Allergy. 59(8):793-805. 2004.
  20. R. H. Green, C E. Brightling, G. Woltmann, D. Parker, A. J. Wardlaw, I. D. Pavord. Analysis of induced sputum in adults with asthma: identification of subgroup with isolated sputum neutrophilia and poor response to inhaled corticosteroids. Thorax. 57(10):875-879, 2002.
  21. Ruth H Green, Christopher E Brightling, Susan McKenna, Beverley Hargadon, Debbie Parker, Peter Bradding, Andrew J Wardlaw, Ian D Pavord. Asthma exacerbations and sputum eosinophil counts: a randomised controlled trial. Lancet. 360(9347):1715-1721, 2002. https://doi.org/10.1016/S0140-6736(02)11679-5
  22. 김창근, 유진호, 고영률, Hirohito Kita. 기도 내 호산구성 염증에서 IL-5와 Eotaxin의 역할. 천식 및 알레르기학회지, 24(1):127-136, 2004.
  23. 김미경. Biology of eosinophil, 대한천석및 알레르기학회 추계학술대회 초록집, 409-417, 1997.
  24. 박해심. 기관지천식에서의 eosinophil cationic protein과 tryptase. 알레르기, 15(4):596-597, 1995.
  25. Rankin, S.M., Conroy, D.M., Williams, T.J. Eotaxin and eosinophil recruitment: implications for human disease. Mol Med Today. 6(1):20-27, 2000.
  26. 김지혜, 오재원, 이하백. 급성 천식 환아에서의 혈청 eosinophil cationic protein치 의 변화에 대한 연구. 알레르기, 16(2):219-229, 1996.
  27. Garlisi, C.G., Kung, T.T., Wang, P., Minnicozzi, M., Umland, S.P., Chapman, R.W., Stelts, D., Crawley, Y., Falcone, A., Myers, J.G., Jones, H., Billah, M.M., Kreutner , W., Egan, R.W. Effects of chronic anti-interleukin-5 monoclonal antibody treatment in a murine model of pulmonary inflammation. Am J Respir Cell Mol Biol 20(2):248-255, 1999.
  28. van Rijt, L.S., Vos, N., Willart, M., Kleinjan， A., Coyle, A.J., Hoogsteden, H.C., Lambrecht, B.N. Essential role of dendritic cell CD80/CD86 costimulation in the induction, but not reactivation, of TH2 effector responses in a mouse model of asthma. J Allergy Clin Immunol. 114(1):166-173, 2004.
  29. Steinman, R.M. The dendritic cell system and its role in immuinogenicity. Ann Rev Immunol 9, 271-296, 1991.
  30. Hart, D.M. Dendritic cells: Unique leukocyte populations which control the primary immune response. Blood. 90(9):3245-3287, 1997.
  31. Banchereau, J., Steinman, R.M. Dendritic cells and the control of immunity. Nature 392(6673), 245-252, 1998.
  32. 조상헌, 장윤석. CpG-ODNs를 이용한 천식의 새로운 면역조절요법. 천식 및 알레르기. 22(1):43-53, 2002.
  33. Sara, M., Rankin, Dolores, M. Conroy and Timothy J. Williams. Eotaxin and eosinophil recruitment: implications for human disease. Molecular Medicine Today. 6(1):20-27, 2000.
  34. 최인선. 동물 천식모형에서 선택적 면역 조절 요법. 천식 및 알레르기. 22(4):663-668, 2002.
  35. Walker, C., Virchow, J.C Jr, Bruijnzeel, P.L., Blaser, K. T cell subsets and their soluble products regulate eosinophilia in allergic and nonallergic asthma. J. Immunol. 146(6):1829-1835, 1991.
  36. Bochner, B.S., Busse, W.W. Advances in mechanisms of allergy. J Allergy Clin Immunol. 113(5):868-875, 2004.
  37. M.J. Koury, J.H. Newman and J.J. Murray, Reversal of hypereosinophilic syndrome and lymphomatoid papulosis with mepolizumab and imatinib. Am J Med 115. 587-589, 2003.
  38. J.K. Garrett, S.C Jameson, B. Thomson, M.H. Collins, L.E. Wagoner, D.K. Freese et al., Anti-interleukin-5(mepolizumab) therapy for hypereosinophilic syndromes. J Allergy Clin Immunol. 113, 115-119, 2004.
  39. Koury, M.J., Newman, J.H., Murray, J.J. Reversal of hypereosinophilic syndrome and lymphomatoid papulosis with mepolizumab and imatinib. Am J Med. 115(7):587-589, 2003.
  40. Garrett, J.K., Jameson, S.C, Thomson, B., Collins, M.H., Wagoner, L.E., Freese, D.K., Beck, L.A., Boyce, J.A., Filipovich, A.H., Villanueva, J.M., Sutton, S.A. Assa'ad AH, Rothenberg ME. Anti-interleukin-5 (mepolizumab) therapy for hypereosinophilic syndromes. J Allergy Clin Immunol. 113(1):115-119, 2004.
  41. 강재성 외. 세포분자면역학(4판). pp198. 258. 서울, 범문사. 2002.
  42. Paul M. O'Byrne, Mark D. Inman and Ellinor Adelroth. Reassessing the Th2 cytokine basis of asthma. TRENDS in Pharmacological Sciences. 25(5):244-248, 2004.
  43. Douglas, S. Robinson. New Therapies for Asthma: Where Next? Pediatric Pulmonology. 36, 369-375, 2003.
  44. Charles A Janeway, Paul Travers, Mark Walport, Mark Shlomchik. Immuobiology. p 473, 481. Gerald. New York. 2001.
  45. S.I. Mayr, R.I. Zuberi, F.-T. Liu. Role of immunoglobulin E and mast cells in murine models of asthma. Brazilian Journal of Medical and Biological Research. 36, 821-827, 2003.
  46. Douglas, S. Robinson. T-cell cytokines: what we have learned from human studies. PAEDIATRIC RESPIRATORY REVIEWS. 5(Suppl A), 553-58, 2004.
  47. G. J. Gleich, H. Kita. Bronchial asthma: Lessons from murine models. Proc. Natl. Acad. Sci. 94, 2101-2102, 1997.
  48. ROB C. AALBERSE: Specific IgE and IgG Responses in Atopic versus Nonatopic Subjects. Am J Respir Crit Care Med. 162, S124-127, 2000.
  49. Rizzo , M. C., A. K. Arruda, M. D. Chapman, E. Fernandez-Caldas, D, Baggio, T. A. E. Platts-Mills and C. K. Naspitz. IgG and IgE antibody responses to dust mite allergens among children with asthma in Brazil. Ann. Allergy. 71, 152-158, 1993.