Genetic Polymorphism of Interleukin 10 Gene and Sasang Constitution in Bell's Palsy Patients

  • Kim, Jong-Won (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Dongeui University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Jung, Tae-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University)
  • Published : 2005.04.25

Abstract

We hypothesized that the IL10 gene is important candidate in the development of Bell's palsy and specific genotypic and allelic variations should be associated with Bell's palsy in the Korean population. In this study, we assessed the SNP (single-nucleotide polymorphism) of IL10 in patients with Bell's palsy. 62 patients with Bell's palsy were selected from the subjects who visited for the Bell's palsy service of the department of acupuncture & moxibustion, college of Oriental Medicine, Daegu Haany University from May 2002 to May 2003. Pyrosequencing was performed for genetic analyses. There was no statistically significant genotypic distribution difference between control and Bell's palsy group And there was not statistically significant allelic frequency difference between control and Bell's palsy group. In this study the IL10 genotypemight not be the risk factor of Bell's palsy patients in Korean. studies will be necessary for the exact genetic markers. Establishment of more systemic approach and high quality of prospective cohorts will be necessary for the good prediction of genetic markers.

Keywords

References

  1. Steiner, L, Mattan, Y. Bell's palsy and herpes viruses: to acyclovir or not to acyclovir? J. Neurol. Sci. 170(1):19-23, 1999.
  2. Yilmaz, M., Tarakcioglu. M., Bayazit, N., Bayazit, Y. A., Namiduru, M., Kanlikarna, M. Serum cytokine levels in Bell's palsy. J Neurol Sci. 197(1-2):69-72, 2002.
  3. Bugawan, T. L., Mirel, D. B., Valdes, A. M., Panelo, A, Pozzilli, P., Erlich, H. A Association and interaction of the IL4R, IL4, and IL13 loci with type 1 diabetes among Filipinos. Am J Hum Genet. 72(6):1505-1514, 2003.
  4. Mirel, D. B., Valdes, A.M., Lazzeroni, L. C., Reynolds, R L., Erlich, H. A, Noble. J. A Association of IL4R haplotypes with type 1 diabetes. Diabetes. 51(11):3336-3341, 2002.
  5. Bidwell, L Keen, L., Gallagher, G., Kimberly, R, Huizinga, T., McDermott, M. et al. Cytokine gene polymorphism in human disease: on-line databases. Genes Immun, 1:3-19, 1999.
  6. Olavesen, M. G., Hampe, L Mirza, M. M., Saiz, R, Lewis, C. M., Bridger, S., Teare, D., Easton, D. F., Herrmann, T., Scott, G., Hirst, J., Sanderson, J., Hodgson, S. V., Lee, L MacPherson, A Analysis of single-nucleotide polymorphisms in the interleukin-4 receptor gene for association with inflammatory bowel disease. Immooogenetics.51(1):1-7, 2000.
  7. Buchs, N., Silzvestri, T., di Ciovine. F. S., Chabaud, M., Vannier, E., Duff, G. W., Miossec, P. IL-4 VNTR gene polymorphism in chronic polyarthritis. The rare allele is associated with protection against destruction. Rheumatology (Oxford). 39(10):1126-1131, 2000.
  8. de Waal Malefyt R, Abrams, L Bennett, B., Figdor, C. G., de Vries, J. E. Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. J Exp Med, 174:1209-1220, 1991.
  9. http://www.pyrosequencing.com
  10. Wieser, F., Fabiani. G., Tempfer, C, Schneeberger, C. Sater, M., Huber, J., Wenzl, R Analysis of an interleukin-6 gene promoter polymorphism in women with endometriosis by pyrosequencing. J Soc Gynecol Investig.10(1):32-36, 2003.
  11. Gruber, J. D., Colligan, P. B., Wolford, J. K. Estimation of single nucleotide polymorphism allele frequency in DNA pools by using Pyrosequencing. Hum Genet. 110(5):395-401, 2002.
  12. Pettersson, M., Bylund, M., Alderborn, A. Molecular haplotype determination using allele-specific PCR and pyrosequencing technology. Genomics.82(3):390-396, 2003.
  13. Pacey-Miller, T., Henry, R Single-nucleotide polymorphism detection in plants using asingle-stranded pyrosequencing protocol with a universal biotinylated primer. Anal Biochem. 317(2):166-170, 2003.
  14. Rowlands,S., Hooper, R, Hughes, R., Burney, P. The epidemiology and treatment of Bell's palsy in the UK. Eur J Neurol. ; 9(1):63-7, 2002.
  15. Murakami. S; Mizobuchi, M., Nakashiro, Y., Doi, T., Hato, N., Yanagihara, N. Bell palsy and herpes simplex virus: identification of viral DNA in endoneurial fluid and muscle. Ann Intern Med. 124(1Pt1):27-30, 1996.
  16. Ohtani, F., Furuta, Y., Horal, P., Bergstrom, T. Rapid strip assay for detection of anti-herpes simplex virus antibodies: application to prediction of varicella-zoster virus reactivation in patients with acute peripheral facial palsy. J Med Virol. 62(1):37-41, 2000.
  17. Morrow, M. J. Bell's Palsy and Herpes Zoster Oticus. Curr Treat Options NeuroI.2(5):407-416, 2000.
  18. Edstrom,S., Hanner, P., Andersen, O., Rosenhall, U., Vahlne, A., Karlsson, B. Elevated levels of myelin basic protein in CSF in relation to auditory brainstem responses in Bell's palsy. Acta Otolaryngol. 103(3-4):198-203, 1987.
  19. Burdin, N., Peronne, C., Banchereau, J., Rousset, F. Epstein? Barr virus transformation induces B lymphocytes to produce human interleukin 10. J Exp Med, 177:295-304, 1993.
  20. Rousset, F., Garcia, E., Defrance, T. et al. Interleukin 10 is a potent growth and differentiation factor for activated human B lymphocytes. Proc Natl Acad Sci, 89:1890-1893, 1992.
  21. Katsikis. P. D., Chu, C. Q., Brennan, F. M., Maini, R. N., Feldmann, M. Immunoregulatory role of interleukin 10 in rheumatoid arthritis. J Exp Med 179:1517-1527, 1994.
  22. Crawley, E., Kay, R., Sillibourne, J., Patel, P., Hutchinson, I., Woo, P. Polymorphic haplotypes of the interleukin-10 5' flanking region determine variable interleukin-10 transcription and are associated with particular phenotypes of juvenile rheumatoid arthritis. Arthritis Rheum, 42:1101-1108, 1999.
  23. Eskdale, J., Keijsers, V., Huizinga, T., Gallagher, G. Microsatellite alleles and single nucleotide polymorphisms (SNP) combine to form four major haplotype families at the human interleukin-10 (IL-10) locus. Genes Immun, 1:151-155, 1999.
  24. Turner, D. M., Williams, D. M., Sankaran, D., Lazarus, M., Sinnott, P. J., Hutchinson, I. V. An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 24:1-8, 1997