The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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5-Hydroxytryptamine Inhibits Glutamatergic Synaptic Transmission in Rat Corticostriatal Brain Slice

  • Cho, Hyeong-Seok (Department of Pharmacology, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Choi, Se-Joon (Department of Pharmacology, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Kim, Ki-Jung (Department of Pharmacology, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Lee, Hyun-Ho (Department of Pharmacology, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Kim, Seong-Yun (Department of Pharmacology, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Cho, Young-Jin (Department of Pharmacology, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Sung, Ki-Wug (Department of Pharmacology, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea)
  • Published : 2005.10.21
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Abstract

Striatum is involved in the control of movement and habitual memory. It receives glutamatergic input from wide area of the cerebral cortex as well as an extensive serotonergic (5-hydroxytryptamine, 5-HT) input from the raphe nuclei. In our study, the effects of 5-HT on synaptic transmission were studied in the rat corticostriatal brain slice using in vitro whole-cell recording technique. 5-HT inhibited the amplitude as well as frequency of spontaneous excitatory postsynaptic currents (sEPSC) significantly, and neither ${\gamma}-aminobutyric$ acid (GABA)A receptor antagonist bicuculline (BIC), nor $N-methyl-_{D}-aspartate$ (NMDA) receptor antagonist, $_{DL}-2-amino-5-phosphonovaleric$ acid (AP-V) could block the effect of 5-HT. In the presence non-NMDA receptor antagonist, 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenxo[f] quinoxaline-7-sulfonamide (NBQX), the inhibitory effect of 5-HT was blocked. We also figured out that 5-HT change the channel kinetics of the sEPSC. There was a significant increase in the rise time during the 5-HT application. Our results suggest that 5-HT has an effect on both pre- and postsynaptic site with decreasing neurotransmitter release probability of glutamate and decreasing the sensitivity to glutamate by increasing the rise time of non-NMDA receptor mediated synaptic transmission in the corticostriatal synapses.

Keywords

References

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