Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Analysis of Gene Eexpression Pattern of Ailanthus altissima Extract and Gleevec on K-562 Leukemia Cell Line

K-562 백혈병 세포주에서 저근백피와 Gleevec을 처리에 의한 유전자 발현 비교 분석

  • Cha, Min-Ho (Department of Medical Research, Korea Institute of Oriental Medicine, School of Lifescience and biothechnology, Korea University) ;
  • An, Won-Gun (College of Dentistry, Pusan National University) ;
  • Jeon, Byung-Hun (College of Oriental Medicine, Wonkwang University) ;
  • Yun, Yong-Gab (College of Oriental Medicine, Wonkwang University) ;
  • Yoon, Yoo-Sik (Department of Medical Research, Korea Institute of Oriental Medicine)
  • 차민호 (한국한의학연구원, 고려대학교 생명과학대학) ;
  • 안원근 (부산대학교 치의과대학) ;
  • 전병훈 (원광대학교 한의과대학) ;
  • 윤용갑 (원광대학교 한의과대학) ;
  • 윤유식 (한국한의학연구원)
  • Published : 2005.08.01
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Abstract

In this study, we investigated gene expression patterns induced by Ailanthus altissima extract and compared it with Gleevec, a well-known anti-leukemia drug, in K562 chromic leukemia cells. Ailanthus altissima extract(100 ug/ml) and Gleevec(50 ug/ml) were treated to cells for 1h, 2h, 4h, and 16h and total RNA was extracted. Gene expressions were evaluated using cDMA microarray, in which 24,000 genes were spotted. Hierarchical clustering analysis showed that expression of genes included in two clusters were increased or decreased time dependently by both Ailanthus altissima extract and Gleevec. Genes included in another cluster were induced by Ailanthus altissima extract but not by Gleevec. In biological process analysis, expression of genes involved in apoptosis, growth arrest and DNA-damage were increased, but genes stimulating cell cycle were decreased. This study provides comprehensive comparison of the patterns of gene expression changes induced by Ailanthus altissima extract and Gleevec in K-562 leukemia cells.

Keywords

References

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