Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Growth Arrest and Apoptosis of Human Uterine Cervical Carcinoma Cells Induced by Trichosanthes Semen Extract

과루인이 자궁경부암세포의 성장억제 및 세포고사에 미치는 영향

  • Lee Jeong Gu (Department of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Kim Yeon Hee (Department of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Lee Dong Nyung (Department of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Kim Hyung Jun (Department of Oriental Gynecology, College of Oriental Medicine, Se-Myung University)
  • 이정구 (세명대학교 한의과대학 부인과학교실) ;
  • 김연희 (세명대학교 한의과대학 부인과학교실) ;
  • 이동녕 (세명대학교 한의과대학 부인과학교실) ;
  • 김형준 (세명대학교 한의과대학 부인과학교실)
  • Published : 2005.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate the effects of Trichosanthes semen extract on the growth and apoptosis of human uterine cervical carcinoma cells. Effects of Trichosanthes semen extract on the growth of ME-180 cells were assayed by MTT assay. Apoptosis induced by Trichosanthes semen extract was detected by fluorescent microscopy, DNA fragmentation analysis and flow cytometry. Caspase-3 and caspase-8 activities were assayed. Trichosanthes semen extract induced ME-180 cells to die in a dose- and time-dependent manner. ME-180 cells treated with Trichosanthes semen extract exhibited typical characteristics of apoptosis. The population of Sub-G1 cells increased significantly, and the cells represented the reduced size, condensed chromatin and apoptotic bodies. They showed the decreased mitochondrial membrane potential and increased activities of caspase-3 and caspase-8. The results suggest that Trichosanthes semen extract induced ME-180 cell apoptosis and the activation of caspase and mitochondrial pathway were involved in the process of Trichosanthes semen extract-induced apoptosis.

Keywords

References

  1. Parkin, D.M., Pisani, P., Ferlay, J. Estimates of the worldwide incidence of eighteen major cancers in 1985. Int J Cancer 54, 594-606, 1993.
  2. 한국중앙암등록본부, 보건복지부. 한국 중앙암등록사업 연례보고서 (2002.1-2002.12). 보건복지부, 2003.
  3. 대한병리학회. 제 3판 병리학. 서울, 고문사. pp 31-38, 896-900, 1998.
  4. 대한산부인과학회 교과서편찬위원회. 부인과학. 서울, 칼빈서적 . pp 980-1027, 1997.
  5. 전국의과대학교수 譯. Current Medical Diagnosis & Treatment. 서울, 한우리 pp 792-793, 1999.
  6. 趙鍾寬. 한방임상종양학. 대전, 周珉出版社. pp 799-818, 2001.
  7. Jonathan S. Berek. Novak's gynecology(12th edition). Williams & Wilkins. pp 1111-1153, 1996.
  8. 韓醫婦人科學 敎材編纂委員會. 韓醫婦人科學(上). 서울, 鼎談. p 18, 319-326, 2001.
  9. 박경미, 김덕님, 김양호, 조한백, 유심근. 濟川煎이 子宮經部癌햄세포(HeLa cell)에 미치는 影響. 大韓韓方婦人科學會誌 16(2):161-176, 2003.
  10. 許浚. 對譯東醫寶鑑, 서울, 法仁文化社. pp 1275-1301, 1999.
  11. 梁秀烈, 李京燮, 宋炳基. 㿂瘕의 東西醫學的 考察. 大韓韓醫學會誌 7(1):84-88, 1986.
  12. 李京燮, 宋炳基. 㿂瘕治方에 關한 文獻考察. 大韓韓醫學會誌 3(2):54-62, 1982.
  13. 陣貴廷, 楊思樹 主編. 實用中西醫結合 診斷治療學(上卷). 서울, 醫聖堂. pp 959-976, 1993
  14. 유심근, 김송백, 박종규, 조한백. 香稜丸이 子宮經部癌세포(HeLa Cell)의 apoptosis에 미치는 影響. 大韓韓方婦人科學會誌 17(2):11-26, 2004
  15. 朴鍾文 등. 六合湯이 子宮經部癌세포(HeLa cell)에 미치는 影響. 大韓韓方婦人科學會誌 16(3):128-146, 2003.
  16. 曺永斗. 斑玄丸과 抗癌劑의 倂用投與가 子宮癌세포(HeLa)에 미치는 影響. 大田大學校 大學院 碩土學位論文, 1995
  17. 巢元方. 巢氏諸病源候論. 서울, 大星出版社. pp 736-738, 1983.
  18. 李慶雨 譯. 編注譯解 黃帝內經靈樞(3卷). 서울, 여강출판사. pp 221-243, 1994.
  19. 孫思遊, 李景榮 등. 備急千金要方校釋. 北京, 人民衛生出版社 pp 258-263, 1998.
  20. 李挻, 安秉國 譯, 國譯編註醫學入門(V卷). 서울, 南山堂. pp 36-43, 2001.
  21. 薛己. 校注婦人良方注釋. 서울, 一中社. pp 157-160, 1989.
  22. 김동철, 백승희, 박종현, 이영선. 香附子가 子宮筋腫세포의 成長抑制와 MAP Kinase 活性 및 Cell Apoptosis에 미치는 影響. 大韓韓方婦人科學會誌 16(2):18-33, 2003.
  23. 李慶雨 譯. 編注譯解 黃帝內經素門(5卷). 서울, 여강출판사. pp 7-160, 1994.
  24. 張介賓, 경희 한의대 제 47기 졸업준비위원회 학술부 校勘. 校勘景岳全書. 서울, 鼎談. pp 439-446, 1999
  25. 龔廷賢. 國譯萬病回春(上卷). 서울, 癸丑文化社. pp 352-361, 1997.
  26. 王淸任. 醫林改錯評譯. 서울, 성보사 pp 129-132, 1998.
  27. 조옥현, 정우석, 서윤점, 조한백. 柴胡疎肝散加味方이 子宮經部癌세포(HeLa cell)에 미치는 影響. 大韓韓方婦人科學會誌 17(4):101-111, 2004
  28. 高昊奎. 加味桂技茯笭丸과 抗癌劑 倂用時 子宮癌세포(HeLa Cell)에 미치는 효과. 大田大學校 大學院, 1995
  29. 金柱延. 生地黃이 子宮經部癌세포(HeLa cell)에 미치는 影響. 圓光大學校 大學院 碩土學位論文, 2003 .
  30. 李允貞 등. 반지련이 자궁암세포(Hela 22918)의 성장 억제에 미치는 영향. 大韓韓方婦人科學會誌12(1):185-196, 1999.
  31. 辛民敎. 臨床本草學. 서울, 永林社. pp 369-372, 1997.
  32. 全國韓醫科大學 本草學敎授 共編著. 本草學. 서울, 永林社. pp 461-463, 1998.
  33. 梁珠勞. 瓜蔞仁의 血管新生 抑制에 대한 硏究. 慶熙大學校 大學院 碩士學位論文, 2003.
  34. Ju Ryoung Lee. Effect of methylene chloride fraction of Trichosanthes kirilowii Maxim on apoptosis in U937 cells. 경희대 동서의학대학원 碩士學位論文, 2004 .
  35. Frank, H., Netter, M.D. CIBA 원색도해의학총서 펀찬위원회 編. The CIBA Collection of Medical Illustrations( II 卷). 서울, 鼎談. pp 170-172, 2000
  36. 목정은 등. 자궁경부암 환자 1058예의 임상적 분석. 대한산부회지 44(1):101-113, 2001
  37. 金昌玟 等. 中藥大辭典. 서울, 鼎談 pp 476-479, 1998
  38. 고경희. Cisplatin에 의한 사람 자궁경부암세포종 HeLa세포의 세포 고사에 미치는 ROS의 영향. 圓光大學校 大學院 博土學{立論文, 2002
  39. 崔益準. 자궁경부암 HaLa 세포주에서 삼산화비소(As2O3)의 세포 고사 기전연구. 圓光大學校 大學院 博土學位論文, 2002
  40. Gao-Liang Ouyang, Qiu-Feng Cai, Min Liu, Rui-Chuan Chen, Zhi Huang, Rui-Sheng Jiang, Fu Chen, Shui-Gen Hong, Shi-Deng Bao. Growth arrest and apoptosis of human hepatocellular carcinoma cells induced byhexamethylene bisacetamide. World j Gastroenterol 10(7): 954-958, 2004.
  41. Shu-Lan Yuan, Yu-Quan Wei, Xiu-Jie Wang, Fei Xiao, Sheng-Fu Lie Zhang. Growth inhibition and apoptosis induction of tanshinone II -A on human hepatocellular carcinoma cells. World J Gastroenterol 10(14):2024-2028, 2004. https://doi.org/10.3748/wjg.v10.i14.2024
  42. Ying ZHANG, Li-jun WU, Shin-ichi TASHIRO, Satoshi ONODERA, Takashi IKEJIMA. Evodiamine induces tumor cell death through different pathways: apoptosis and necrosis. Acta Pharmacol Sin 25(1):83-89, 2004.
  43. Kang, Y.J., Chaen, Y., Epstein, P.N. Suppression of doxorubicin cardiotoxicity by overexpression of catalase in the heart of transgenic mice. J Biol Chem 271(21): 12610-12616, 1996.
  44. Rocha, G.M. et al. Direct toxicity of nonsteroidal antiinflammatory drugs for renal medullary cells. Proc Natl Acad Sci USA 98(9):5317-5322, 2001.
  45. 강신성 등. 생물과학. 아카데미서적 pp 292-304, 1997.
  46. 李和京. 鷷血藤이 子宮筋腫세포의 增殖抑制와 세포自滅死 관련 遺傳子의 發顯에 미치는 影響. 대구한의대학교 대학원 博土學位論文, 2004
  47. 전미혜, 백승희, 신상우, 이영선. 五積散이 子宮筋腫세포의 死藏과 Cell Apoptosis에 미치는 影響. 大韓韓方婦人科學會誌 16(2):45-55, 2003.
  48. Sugawara, T. et al. Overexpression of copper/zinc superoxide dismutase in transgenic rats protects vulnerable neurons against ischemic damage by blocking the mitochondrial pathway of caspase activation. J Neurosci 22(1):209-217, 2002
  49. Sanchez-Alcazar, J.A., Khodjakov, A., Schneider, E. Anticancer drugs induce increased mitochondrial cytochrome c expression that precedes cell death. Cancer Res 61(3):1038-1044, 2001.
  50. Hollwnsworth, S.B. et al. Glial cell type-specific responses tio menadione-induced oxidative stress, Free Radic Biol Med 28(8):1161-1174, 2000.
  51. Le, X.F. et al. Recombinant PML adenovirus suppresses growth and tumorigenicity of humman breast cancer cells by inducing G1 cell cycle arrest and apoptosis. Oncogene 16(14):1839-1849, 1998.
  52. Musgrove, E.A. et al. Cyclin D1 induction in brreast cancer cells shortens G1 and is sufficiant for cells arrested in G1 to complete the cell cycle. Proc Natl Acad Sci USA 91(17):8022-8026, 1994.
  53. Nigg, EA Cyclin-dependent protein kinases: key regulators of the eukaryotic cell cycle. Bioessays 17(6):471-480, 1995.
  54. 박소월, 백승희. 鹿茸이 雌雖性생쥐의 生殖能力과 Caspase-3 MAP kinase 및 MPG 遺傳子 發顯에 미치는 影響. 大韓韓方婦人科學會誌17(1):1-14, 2004.
  55. Perry, D.K. et al. Zinc is a potent inhibit or protease, caspase-3. A novel target for zinc in the inhibition of apoptosis. J Biol Chem 272, 18530-18533, 1997.
  56. Van Loo G et al. the role of mitochondrial factors in apoptosis: a Russian roulette with more than one bullet: Cell death and Differentiation Shared pathways: death receptors and cytotoxic drugs in cancer therapy. Petak I, Houghton JA Pathol, Oncol. Res 9(1):1031-1042, 2002.
  57. Wei-wei An, Min-wei wang, Shin-ichi Tashiro, Satoshi Ondera, Takashi Ikejima. Norcantharidin Induces Human Melanoma A375-S2 Cell Apoptosis through Mitochondrial and Caspase Pathways. J Korean Med Sci 19:560-566, 2004
  58. Susin, S.A et al. Bcl-2 inhibits the mitochondrial release of an apoptotic protease. J. Exp. Med 184(4):1331-1341, 1996
  59. Zamazmi, N. et al. Mitochondrial control of nuclear apoptosis. J. Exp. Med 183(4):1533-1544, 1996.
  60. Zanzani, N. et al. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. J. Exp. Med 182(2):367-377, 1995.
  61. Castedo et al. Mitochondrial pertubation define lymphocytes undergoing apoptotic deplectin in vivo. Eur. J. Immunol pp 3277-3284, 1995.